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Radiation and Environmental Biophysics

, Volume 24, Issue 4, pp 303–314 | Cite as

The relationship between sensitivity to 60-Hz electric fields and induced transmembrane potentials in plants root cells

  • M. Inoue
  • M. W. Miller
  • E. L. Carstensen
  • A. A. Brayman
Article

Summary

Growth rates and cell diameters were determined from 12 species of plant roots exposed to a 60-Hertz (Hz) electric field of 360 Volts per meter (V/m) in an aqueous inorganic nutrient medium [conductivity: 0.07–0.09 Siemens per meter (S/m)]. The degree of growth depression ranged from zero to nearly 100 percent of control. Cell diameters ranged from 13.5 to 31.8 µm as an averaged value for procambial, cortical, and meristem cells. Sensitivity to the electric field as determined by root growth rate reduction increased with increasing cell size. Sensitivity also increased with increase in 60 Hz induced transmembrane potentials; the transmembrane potential threshold for growth reduction was about 6.0 mV and the potential for near-complete cessation of growth was about 10–11 mV.

Two different hypothetical mechanisms of action by which applied electric fields induce biological effects at the cellular level were tested. The two mechanisms pertain to different possible modes of action of applied electric fields: one mechanism postulates the involvement of the transmembrane field, the other mechanism postulates the tangential electric field as the important factor for inducing biological effects. The data support the transmembrane and not the tangential field mechanism. It is concluded that the effects observed are consistent with a membrane related mechanism and that there is a narrow range (a few mV) between threshold and debilitating induced membrane potentials.

Keywords

Applied Electric Field Inorganic Nutrient Meristem Cell Cell Diameter Growth Rate Reduction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • M. Inoue
    • 1
  • M. W. Miller
    • 2
  • E. L. Carstensen
    • 3
  • A. A. Brayman
    • 2
  1. 1.Radiation Center of Osaka PrefectureShinke, Sakai, OsakaJapan
  2. 2.Department of Radiation Biology and Biophysics, School of Medicine and DentistryThe University of RochesterRochesterUSA
  3. 3.Department of Electrical EngineeringThe University of RochesterRochesterUSA

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