Radiation and Environmental Biophysics

, Volume 24, Issue 4, pp 239–249 | Cite as

Oxygen effect and influence of buffer on radiation-induced tritium cleavage from phage-T2-DNA-(methyl-3H) in aqueous solution

  • O. Merwitz
  • W. Köhnlein


The effect of gamma irradiation on tritium release into water (HTO) from phage T2-DNA-(methyl-3H) was studied in diluted aqueous solution. The influence of O2 and of citrate and phosphate buffer was investigated. In oxygenated solutions an enhancement ratio of 3 was found. From the linear dose yield relationsG values were calculated. In the presence of N2O tritium release was doubled, whereas tritium release decreased with increasing citrate concentration. It has been concluded that the major precursor of this tritium release is the OH radical. This was substantiated by experiments using thymine-(methyl-3H) and dimethylsulfoxide.


Oxygen Phosphate Aqueous Solution Phosphate Buffer Citrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Achey PM, Duryea HZ, Michaels GS (1974) Choice of solvent for studying the role of water in ionizing radiation action on DNA. Radiat Res 58:83–90PubMedGoogle Scholar
  2. Blok J, Verheij WSD (1968) The attack of free radicals on biologically active DNA in irradiated aqueous solutions. Radiat Res 34:689–703PubMedGoogle Scholar
  3. Burr JG (1962) IAEA Tritium Symposium, Vol 1, pp 137–151, ViennaGoogle Scholar
  4. Das S, Deeble DJ, von Sonntag C (1985) Site of H atom attack on uracil and its derivatives in aqueous solution. Z Naturforsch 40c (in press)Google Scholar
  5. Ewing D (1983) Radiation sensitization of E. coli B/r by nitrous oxide. Radiat Res 96:275–283PubMedGoogle Scholar
  6. Farhataziz, Ross AB (1977) Selected specific rates of reaction of transients from water in aqueous solution. III Hydroxyl radical and perhydroxyl radical and their radical ions. NSRDS-NBS 59 National Bureau of Standards, Washington USAGoogle Scholar
  7. Fujita S, Steenken S (1981) Pattern of OH radical addition to uracil and methyl- and carboxyl-substituted uracils. Electrontransfer of OH adducts with N,N,N′,N′-tetramethyl-p-phenylendiamine and tetranitromethane. J Am Chem Soc 103:2540–2545Google Scholar
  8. Held KD, Wahl-Synek R, Powers EL (1978) Radiation sensitivity of transforming DNA. Int J Radiat Biol 33:317–324Google Scholar
  9. Janata E, Schuler RH (1982) Rate constants for scavenging eaq in N2O-saturated solutions. J Phys Chem 86:2078–2084Google Scholar
  10. Jortner J, Ottolenghi M, Rabani J, Stein G (1962) Conversion of solvated electrons into hydrogen atoms in the photo- and radiation chemistry of aqueous solutions. J Chem Phys 37:2488–2495Google Scholar
  11. Köhnlein W, Merwitz O (1983) Gamma-induced hydrogen cleavage and demethylation of position-labeled DNA and its precursors. Proc 7th Intern Congr Radiat Res, Amsterdam, pp A3–21Google Scholar
  12. Lafleur MVM, Loman H, Blok J (1975) On the role of phosphate in the irradiation of DNA in aqueous solutions. Int J Radiat Biol 27:197–200Google Scholar
  13. Melander L, Saunders WH Jr (1980) Reaction rates of isotopic molecules. Wiley, New YorkGoogle Scholar
  14. Merwitz O, Köhnlein W (1982) Gamma-induced demethylation of T2 DNA-(thymidine-methyl-14C) in deoxygenated aqueous solution. 5th Symposium on Radiation Chemistry, Siofok. Publishing House Hung Acad Sci, Budapest, pp 1045–1051Google Scholar
  15. Merwitz O, Otto R (1974) Linear and sigmoid dose-effect relations: investigations with gamma-irradiated thymine(methyl-3H) in aqueous solution and in the solid state. Radiat Environ Biophys 11:69–77PubMedGoogle Scholar
  16. Mitchel REJ, Morrison DP (1983) A comparison between rates of cell death and DNA damage during irradiation of Saccharomyces cerevisiae in N2 and N2O. Radiat Res 96:374–379PubMedGoogle Scholar
  17. Powers EL, Cross M (1970) Nitrous oxide as a sensitizer of bacterial spores to X-rays. Int J Radiat Biol 17:501–514Google Scholar
  18. Pryor WA (1966) Free radicals. Academic Press, New YorkGoogle Scholar
  19. Rijn K van, Mayer T, Blok J, Verberne JB, Loman H (1985) Reaction rates of OH radicals withφ X 174 DNA: Influence of salt and scavengers. Int J Radiat Biol 47:309–317Google Scholar
  20. Roots R, Chatterjee A, Blakely E, Chang P, Smith K, Tobias C (1982) Radiation responses in air, nitrous oxide-, and nitrogen-saturated mammalian cells. Radiat Res 92:245–254PubMedGoogle Scholar
  21. Roti Roti JL, Cerutti PA (1974) Gamma-ray induced thymine damage in mammalian cells. Int J Radiat Biol 25:413–417Google Scholar
  22. Schuchmann HP, Wagner R, Sonntag C von (1985) to be publishedGoogle Scholar
  23. Smith JD, Wyatt GR (1951) The composition of some microbial deoxypentose nucleic acids. Biochem J 49:144–148Google Scholar
  24. Sonntag C von, Hagen U, Schön-Bopp A, and Schulte-Frohlinde D (1981) Radiation-induced strand breaks in DNA: Chemical and enzymatic analysis of end groups and mechanistic aspects. Adv Radiat Biol 9:103–142Google Scholar
  25. Swinehart JL, Cerutti PA (1975) Gamma-ray induced thymine damage in the DNA in coli phageφ X 174 and in E. coli. Int J Radiat Biol 27:83–94Google Scholar
  26. Ward JF (1975) Molecular mechanisms of radiation induced damage to nucleic acids. Advances Radiat Biol 5:181–239Google Scholar
  27. Ward JF, Kuo I (1976) Strand breaks base release and postirradiation changes in DNA gamma irradiated in dilute oxygen-saturated aqueous solution. Radiat Res 60:485–498Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • O. Merwitz
    • 1
    • 2
  • W. Köhnlein
    • 1
    • 2
  1. 1.Institut für Chemie 1 (Nuklearchemie) der Kernforschungsanlage Jülich GmbHJülichFederal Republic of Germany
  2. 2.Institut für Strahlenbiologie der Universität MünsterMünsterFederal Republic of Germany

Personalised recommendations