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Radiation and Environmental Biophysics

, Volume 24, Issue 4, pp 239–249 | Cite as

Oxygen effect and influence of buffer on radiation-induced tritium cleavage from phage-T2-DNA-(methyl-3H) in aqueous solution

  • O. Merwitz
  • W. Köhnlein
Article

Summary

The effect of gamma irradiation on tritium release into water (HTO) from phage T2-DNA-(methyl-3H) was studied in diluted aqueous solution. The influence of O2 and of citrate and phosphate buffer was investigated. In oxygenated solutions an enhancement ratio of 3 was found. From the linear dose yield relationsG values were calculated. In the presence of N2O tritium release was doubled, whereas tritium release decreased with increasing citrate concentration. It has been concluded that the major precursor of this tritium release is the OH radical. This was substantiated by experiments using thymine-(methyl-3H) and dimethylsulfoxide.

Keywords

Oxygen Phosphate Aqueous Solution Phosphate Buffer Citrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • O. Merwitz
    • 1
    • 2
  • W. Köhnlein
    • 1
    • 2
  1. 1.Institut für Chemie 1 (Nuklearchemie) der Kernforschungsanlage Jülich GmbHJülichFederal Republic of Germany
  2. 2.Institut für Strahlenbiologie der Universität MünsterMünsterFederal Republic of Germany

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