Abstract
Copper-zinc superoxide dismutase fromAscaris suum (Nematoda) was purified in a new, more efficient, and faster manner. The process included differential centrifugation, fractionation with ammonium sulfate, and sodium dodecyl sulfate-polyacrylamide electrophoresis, yielding a 340-fold purification (specific activity of 47 units/mg). Optimal storage conditions, optimal pH range, thermostability, molecular weight and ultravioltet-visible absorption spectrum of the enzyme are described, and a new enzymatic model for pharmacological screening is suggested.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- (SOD):
-
Superoxide dismutase
- (EDTA):
-
Ethylenediaminetetraacetic acid
- (SDS):
-
Sodium dodecyl sulfate
- (NBT):
-
p-nitrotetrazolium blue
- (UV):
-
Ultraviolet
References
Callahan, H. L., Crough, R. K. and James, E. R. (1988)Parasitol. Today 4:218–225.
Kazura, J. W. and Meshnick, S. R. (1984)Mol. Biochem. Parasitol. 10:1–10.
Bryant, C. and Behm, C. A. (1989)Biological Adaptation in Parasites Chapman & Hall Ltd, London pp. 259.
Warren, K. S. (1988) in:The Biology of Parasitism (Englund, P. T. and Sher, A., eds) Alan R. Liss Inc., New York. pp. 3–12.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951)J. Biol. Chem. 193:265–275.
Laemmli, U. K. (1970)Nature 227:680–685.
Ayala, F. J., Powell, J. R., Tracey, M. L., Mourao, C. A. and Perez-Salas, F. (1972)Genetics 70:113–119.
McCord, J. M. and Fridovich, I. (1969)J. Biol. Chem. 244:6049–6055.
Bannister, W. H., Anastasi, A. and Bannister, J. V. (1977) in:Superoxide and Superoxide Dismutases. (Michelson, A. M., McCord, J. M. and Fridovich, I., eds) Academic Press, New York, pp. 107–128.
Randall, L., Weselake, S. L., Chesney, A. P. and Friesen, A. D. (1986)Anal. Biochem. 155:193–197.
Smith, N. C. and Bryant, C. (1986)Int. J. Parasitol. 16:617–622.
Sanchez-Moreno, M., Leon, P., Monteoliva, M. and Garcia-Ruiz, M. A. (1988)Comp. Biochem. Physiol. 89:521–523.
Paul, J. M. and Barrett, J. (1980)Int. J. Parasitol. 10:121–124.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sanchez-Moreno, M., Entrala, E., Janssen, D. et al. Copper-zinc superoxide dismutase fromAscaris suum (Nematoda): Purification and characterization. Biosci Rep 14, 83–90 (1994). https://doi.org/10.1007/BF01210303
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01210303