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Studies on tumor-cell-induced platelet aggregation in human lung cancer cell lines

  • Original Paper
  • Experimental Oncology
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Abstract

We investigated the ability of human lung cancer cells of different histological subtypes to cause platelet aggregation. Tumor-cell-induced platelet aggregation (TCIPA) was studied in vitro in 13 human lung cancer cell lines [small-cell lung cancer (SCLC), squamous-cell lung cancer, large-cell lung cancer, adenocarcinoma and alveolar-cell lung cancer]. Three tumor cell lines failed to aggregate platelets in plateletrich plasma, whereas platelet aggregation was induced by 12 cell lines when added to washed platelets and minimal amounts of platelet-poor plasma (0.5% v/v). The thrombin antagonist hirudin inhibited TCIPA in non-small-cell lung cancer cell lines (NSCLC). In SCLC, TCIPA was fully abolished only when the ADP scavenger apyrase was added to hirudin. Thus ADP and thrombin generation by these tumor cell lines are responsible for platelet aggregation. The ability to activate platelets independently of coagulation factors VII and X was demonstrated for 8 cell lines. Electronmiicroscopically, direct tumor-cell/platelet contact was found to be the initiating mechanism of TCIPA in SCLC, whereas tumor-cell/platelet contacts in NSCLC could only be observed at the peak of the aggregation curve. Lung cancer cells activate platelets in vitro by generation of thrombin and/or ADP.

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Abbreviations

TCIPA :

tumor-cell-induced platelet aggregation

SCLC :

small-cell lung cancer

NSCLC :

non-small-cell lung cancer

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Authors and Affiliations

  1. Department of Surgery, University Clinic of Regensburg, Franz-Josef-Strauß Allee 11, 93053, Regensburg, Germany

    Ernst Heinmöller & Hubert Zirngibl

  2. Department of Surgery, Friedrich-Schiller University Jena, Germany

    Rolf J. Weinel

  3. Department of Internal Medicine, Philipps-University Marburg, Germany

    Hans H. Heidtmann

  4. Paul-Ehrlich-Institute Langen, Germany

    Ursula Salge & Reiner Seitz

  5. Institute of Pathology, University Clinic Bergmannsheil, Bochum, Germany

    Inge Schmitz & Klaus M. Müller

Authors
  1. Ernst Heinmöller
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  2. Rolf J. Weinel
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  3. Hans H. Heidtmann
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  4. Ursula Salge
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  7. Klaus M. Müller
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  8. Hubert Zirngibl
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Heinmöller, E., Weinel, R.J., Heidtmann, H.H. et al. Studies on tumor-cell-induced platelet aggregation in human lung cancer cell lines. J Cancer Res Clin Oncol 122, 735–744 (1996). https://doi.org/10.1007/BF01209121

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  • DOI: https://doi.org/10.1007/BF01209121

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