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Phosphoserine phosphatase of human brain: Partial purification, characterization, regional distribution, and effect of certain modulators including psychoactive drugs

Neurochemical Research volume 15, pages 1203–1210 (1990)Cite this article

Abstract

Phosphoserine phosphatase (PSPase), a cytosolic enzyme has been purified 106 fold from human brain, by employing conventional protein purification techniques. The use of MgCl2 (10 mM) and chloroform treatment, during purification enabled the removal of non-specific proteins. The final enzyme preparation exhibited a broad pH optimum of 5.6–6.6 and could dephosphorylate bothl andd enantiomers of the phosphoserine, but with different Km values for O-P-L serine (3.6×10−5M) and O-P-D serine (1×10−4M). Enzyme activity was found to be specific for phosphoserine, whereas other phosphoesters including phosphothreonine and phosphoproteins such as casein and phosvitin were found to be poor substrates. The enzyme activity was uncompetitively inhibited byl-serine. Further the PSPase activity was inhibited by vanadate, (41%), trifluoperazine (23%), chlorpromazine (34%) at an equimolar concentration of 1 mM, whereas lithium and ethanol did not influence the enzyme activity. Minor tranquilizers such as diazepam and chlordiazepoxide activated the enzyme activity to an extent of 13% and 59% respectively. In addition, species and regionwise heterogeneity was observed with respect to distribution of enzyme activity in six major areas of human, rabbit and rat brains.

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Affiliations

  1. Department of Neurochemistry, National Institute of Mental Health and Neuro Sciences, 560 029, Bangalore, India

    Veeranna & K. Taranath Shetty

  2. UGC Teacher fellow, Government of India, India

    Veeranna

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  1. Veeranna
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  2. K. Taranath Shetty
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Veeranna, Shetty, K.T. Phosphoserine phosphatase of human brain: Partial purification, characterization, regional distribution, and effect of certain modulators including psychoactive drugs. Neurochem Res 15, 1203–1210 (1990). https://doi.org/10.1007/BF01208581

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  • DOI: https://doi.org/10.1007/BF01208581

Key Words

  • Phosphoserine phosphatase
  • purification
  • modulators
  • Regional distribution