Skip to main content
Log in

Separation of sperm and blood cells during isolation of motile human spermatozoa

  • Short Communications
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Silber SJ: Results of microsurgical vasoepididymostomy: Role of epididymis in sperm maturation. Hum Reprod 1989;4:298–303

    PubMed  Google Scholar 

  2. Silber SJ, Balmaceda J, Borrero C, Ord T, Asch R: Pregnancy with sperm aspiration from the proximal head of the epididymis: A new treatment for congenital absence of the vas deferens. Fertil Steril 1988;50:525–528

    PubMed  Google Scholar 

  3. Silber SJ, Ord T, Balmaceda J, Patrizio P, Asch RH: Congenital absence of the Vas Deferens. N Engl J Med 1990;323:1788–1792

    PubMed  Google Scholar 

  4. Ord T, Marello E, Patrizio P, Balmaceda JP, Silber SJ, Asch RH: The role of the laboratory in the handling of epididymal sperm for assisted reproductive technologies. Fertil Steril 1992;57:1103–1106

    PubMed  Google Scholar 

  5. Morales P, Vantman D, Barros C, Vigil P: Human spermatozoa selected by Percoll gradient or swim-up are equally capable of binding to the human zona pellucida and undergoing the acrosome reaction. Hum Reprod 1991;6:401–404

    PubMed  Google Scholar 

  6. Gorus FK, Pipeleers DG: A rapid method for the fractionation of human spermatozoa according to their progressive motility. Fertil Steril 1981;35:662–665

    PubMed  Google Scholar 

  7. Bolton VN, Warren RE, Braude PR: Removal of bacterial contaminants from semen for in vitro fertilization or artificial insemination by the use of buoyant density centrifugation. Fertil Steril 1986;46:1128–1132

    PubMed  Google Scholar 

  8. Van Der Zwalmen P, Bertin-Segal G, Geerts L, Debauche C, Schoysman R: Sperm morphology and IVF pregnancy rate: Comparison between Percoll gradient centrifugation and swim-up procedures. Hum Reprod 1991;6:581–588

    PubMed  Google Scholar 

  9. Guérin JF, Mathieu C, Lornage J, Pinatel MC, Boulieu D: Improvement of survival and fertilizing capacity of human spermatozoa in an IVF programme by selection on discontinuous Percoll gradients. Hum Reprod 1989;4:798–804

    PubMed  Google Scholar 

  10. Browne ES, Sohal GS, Bhalla VK: Characterization of functional leydig cells after purification on a continuous gradient of Percoll. J Androl 1990;11:379–389

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ziebe, S., Andersen, C.Y. Separation of sperm and blood cells during isolation of motile human spermatozoa. J Assist Reprod Genet 10, 175–176 (1993). https://doi.org/10.1007/BF01207745

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01207745

Keywords

Navigation