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Co-optimization of kinetic ceruloplasmin determination based on response surface methodology

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Co-Optimization of Kinetic Ceruloplasmin Determination Based on Response Surface Methodology

The kinetic determination of the enzyme ceruloplasmin in blood serum by its peroxidase-like activity onπ-phenylenediamine6 is revisited by means of response surface methodology. This investigation technique revealed the most important interactions of ceruloplasmin with the variables pH, substrate and buffer concentration. As the result a method of improved precision and accuracy is proposed for clinical determinations of the enzyme in the physiologically relevant concentration range between 0.01 and 1 g/l.

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Authors and Affiliations

  1. Department of Chemistry, Bergakademie Freiberg, GDR

    M. Otto

  2. Hospital Wurzen, GDR

    G. Kaftan

  3. Department of Chemistry, Karl-Marx-University Leipzig, GDR

    G. Werner

Authors
  1. M. Otto
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  2. G. Kaftan
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  3. G. Werner
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Otto, M., Kaftan, G. & Werner, G. Co-optimization of kinetic ceruloplasmin determination based on response surface methodology. Mikrochim Acta 86, 417–430 (1985). https://doi.org/10.1007/BF01206911

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  • DOI: https://doi.org/10.1007/BF01206911

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