Skip to main content
SpringerLink
Log in

Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction-tools for probing lipopolysaccharide and lipid a membranes from gram-negative bacteria

  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

FT-IR and X-ray techniques were used to get an insight into the structure of synthetic Salmonella lipid A membranes. While FT-IR was sensitively providing structure information at the molecular level from different parts of the molecule, X-ray scattering gave data on long-range order phenomena and on fatty acid chain packing pattern. The lipid A membranes exhibited lamellar structures throughout the temperature range of 4°C to 60°C and showed up to three temperature induced “transitions”. The main transitionT 3 at 47°C was assigned to anL β−L α transition. The weak features atT 1 andT 2 localized between 15 and 40°C seemed to be connected to packing phenomena of lipid A aggregates within the distorted lattice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. H. Nikaido, T. Nakae,Adv. Microb. Physiol. 1979,20, 163.

    Google Scholar 

  2. H. Labischinski, G. Barnickel, H. Bradaczek, D. Naumann, E. T. Rietschel, P. Giesbrecht,J. Bacterial. 1985,162, 9.

    Google Scholar 

  3. E. Th. Rietschel, C. Galanos, O. Lüderitz, O. Westphal, in:Immunopharmacology and the Regulation of Leukocyte Function (D. R. Webb, ed.), Marcel Dekker, Basel, 1982, p. 183.

    Google Scholar 

  4. D. Naumann, Ch. Schultz, J. Born, H. Labischinski, U. Seydel,Eur. J. Biochem. 1987,164, 159.

    Google Scholar 

  5. K. Brandenburg, U. Seydel,Biochim. Biophys. Acta 1985,775, 225.

    Google Scholar 

  6. R. T. Coughlin, A. A. Peterson, A. Haug, J. J. Pownall, E. J. McGroarty,Biochim. Biophys. Acta 1985,821, 404.

    Google Scholar 

  7. H. L. Casal, H. H. Mantsch,Biochim. Biophys. Acta 1984,779, 381.

    Google Scholar 

  8. A. Tardieu, V. Luzzati, F. C. Renan,J. Mol. Biol 1973,75, 711.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Robert-Koch-Institute of the Federal Health Office, Nordufer 20, D-1000, Berlin 65

    Dieter Naumann & Harald Labischinski

  2. Institute of Crystallography, Free University Berlin, Takustrasse 6, D-1000, Berlin 33

    Christian Schultz, Jens Born & Fritz Rogge

Authors
  1. Dieter Naumann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christian Schultz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jens Born
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fritz Rogge
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Harald Labischinski
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Naumann, D., Schultz, C., Born, J. et al. Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction-tools for probing lipopolysaccharide and lipid a membranes from gram-negative bacteria. Mikrochim Acta 94, 379–383 (1988). https://doi.org/10.1007/BF01205911

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01205911

Key words

Search

Navigation