Pushing the limits of quantitative FTIR-microscopy

Abstract

The quantitative analysis of organic microsamples [vinylite (PVC-PVA) cellulose-nitrate and polyamide] on Al-foils was performed with good precision (rel. standard deviation 1.04% to 12.1%) after calibration of their main IR-bands with relative conductometric techniques. It was shown that the lower detection limit using the 15×Cassegrain objective was 1μm and that the linear range of the calibration curves was limited by a deviation of Beer's law atA>1.2 due to the “reststrahlen-effect”.