Abstract
Currently, much effort is being directed to the determination of the three-dimensional structure of proteins. Two classes of research are of interest; spectrometric techniques which include Fourier transform infrared (FT-IR) spectrometry, and non-spectrometric prediction schemes. The spectra obtained using FT-IR spectrometry, are analyzed to determine the percentages of alpha-helices, beta-pleated sheets, and non-structured coils in a protein. Unfortunately, FT-IR, as well as other spectrometric techniques, cannot be used to determine the exact secondary structure of a protein reliably. Non-spectrometric prediction methods yield information on the exact secondary structure, but are not always accurate. Most prediction methods relate the primary amino acid sequence to the secondary structure of a protein, allowing sequential secondary structure information for the protein examined to be obtained. The goal of this research is to incorporate FT-IR with a prediction method, resulting in an improvement in the accuracy of the prediction.
Similar content being viewed by others
References
J. A. de Haseth, T. L. Isenhour, An Information Theoretical Approach to the Determination of the Secondary Structure of Globular Proteins, in:Computer Assisted Structure Elucidation (D. H. Smith, ed.),ACS Sym. Ser. 1977,54, 46.
D. M. Byler, H. Susi,Biopolymers 1986,25, 469.
P. Y. Chou, G. C. Fasman,Biochemistry 1974,13, 211.
B. W. Matthews,Biochim. Biophys. Acta 1975,405, 442.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tripodi, E.V., de Haseth, J.A. Incorporation of FT-IR spectral data in a computer-assisted prediction of globular protein structure. Mikrochim Acta 94, 121–124 (1988). https://doi.org/10.1007/BF01205852
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01205852