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Treatment with gonadotropins impaired implantation and fetal development in mice

Journal of Assisted Reproduction and Genetics volume 10, pages 286–291 (1993)Cite this article

Purpose

The effect of gonadotropins on implantation and fetal development in mice was investigated by superovulation with pregnant mare serum gonadotropin and human chorionic gonadotropin. In a previous study fetal growth was found to be highly retarded.

Results

Assessment of implantation in vivo revealed that late implantation did occur. Gestational length was highly extended, the mean number of live fetuses per pregnant mouse was lower and their mean weight significantly reduced. In vitro experiments revealed no significant difference in the rate of blastocyst adhesion and trophoblast outgrowth development. Immunohistochemical staining, however, showed that blastocysts from superovulated mice had smaller trophoblastic outgrowths than control embryos. Staging embryonic development at the time of flushing, however, revealed retarded embryo development in vivo in hormone-treated mice. After correlation with embryonic stage at the beginning of the culture, there was no difference in the size of trophoblastic outgrowths.

Conclusion

Treatment with gonadotropins impaired implantation and embryonic/fetal development. Changes in maternal milieu, rather than in embryo quality, may be responsible for the adverse effects observed.

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References

  1. Ertzeid G, Storeng R: Adverse effects of gonadotropin treatment on pre- and postimplantation development in mice. J Reprod Fert 1992;96:649

    Google Scholar 

  2. Doyle P, Beral V, Maconochie: Preterm delivery, low birthweight and small-for-gestational-age in liveborn singleton babies resulting from in-vitro fertilization. Hum Reprod 1992;3:425

    Google Scholar 

  3. Elbling L, Colot M: Abnormal development and transport and increased sister chromatid exchange in preimplantation embryos following superovulation in mice. Mutat Res 1985;147:189

    PubMed  Google Scholar 

  4. Elbling L, Colot M: Persistence of SCE-inducing damage in mouse embryos and fetuses following superovulation. Mutat Res 1989;76:117

    Google Scholar 

  5. Fossum GF, Davidson A, Paulson RJ: Ovarian hyperstimulation inhibits embryo implantation in the mouse. J Vitro Fert Embryo Transfer 1989;6:7

    Google Scholar 

  6. Miller BG, Armstrong DT: Infertility in superovulated immature rats: Role of ovarian steroid hypersecretion. Biol Reprod 1982;26:861

    PubMed  Google Scholar 

  7. McRae AC, Church RB: Cytoplasmic projections of trophectoderm distinguish implanting from preimplanting and implantation-delayed mouse blastocysts. J Reprod Fert 1990;88:31

    Google Scholar 

  8. Whittingham DG: Embryo banks in the future of developmental genetics. Genetics 1974;78:395

    PubMed  Google Scholar 

  9. Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonald S, Pulford KAF, Stein H, Mason DY: Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 1984;32:212

    Google Scholar 

  10. Wilson ED, Edwards RG: Parturition and increased litter size in mice after superovulation. J Reprod Fert 1963;5:179

    Google Scholar 

  11. Fowler RE, Edwards RG: Induction of superovulation and pregnancy in mature mice by gonadotrophins. J Endocrinol 1957;15:374

    PubMed  Google Scholar 

  12. de Boer P, van dere Hoeven FA, Wolters EMTJ, Mattheij JAM: Embryo loss, blastomere development and chromosome constitution after human chorionic gonadotropin-induced ovulation in mice and rats with regular cycles. Gynecol Obstet Invest 1991;32:200

    PubMed  Google Scholar 

  13. Sappino AP, Huarte J, Belin D, Vassali JD: Plasminogen activators in tissue remodeling and invasion: mRNA localization in mouse ovaries and implanting embryos. J Cell Biol 1989;109:2471

    PubMed  Google Scholar 

  14. Champlin AK, Kuzia SJ, Rice BA, Mobraaten LE: Cell surface characteristics of blastocysts from spontaneously ovulating and gonadotropin-treated mice. Biol Reprod 1987;36:439

    PubMed  Google Scholar 

  15. Walton EA, Huntly S, Kennedy TG, Armstrong DT: Possible causes of implantation failure in superovulated immature rats. Biol Reprod 1982;27:847

    PubMed  Google Scholar 

  16. Pollard JW: Regulation of polypeptide growth factor synthesis and growth factor-related gene expression in the rat and mouse uterus before and after implantation. J Reprod Fert 1990;88:721

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Ullevaal University Hospital, 0407, Oslo, Norway

    G. Ertzeid & R. Storeng

  2. Department of Surgery, Ullevaal University Hospital, 0407, Oslo, Norway

    T. Lyberg

Authors
  1. G. Ertzeid
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  2. R. Storeng
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  3. T. Lyberg
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Ertzeid, G., Storeng, R. & Lyberg, T. Treatment with gonadotropins impaired implantation and fetal development in mice. J Assist Reprod Genet 10, 286–291 (1993). https://doi.org/10.1007/BF01204944

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  • DOI: https://doi.org/10.1007/BF01204944

Key words

  • gonadotropins
  • late implantation
  • embryo/fetal development
  • plasminogen activator
  • mouse