Treatment with gonadotropins impaired implantation and fetal development in mice
- 66 Downloads
The effect of gonadotropins on implantation and fetal development in mice was investigated by superovulation with pregnant mare serum gonadotropin and human chorionic gonadotropin. In a previous study fetal growth was found to be highly retarded.
Assessment of implantation in vivo revealed that late implantation did occur. Gestational length was highly extended, the mean number of live fetuses per pregnant mouse was lower and their mean weight significantly reduced. In vitro experiments revealed no significant difference in the rate of blastocyst adhesion and trophoblast outgrowth development. Immunohistochemical staining, however, showed that blastocysts from superovulated mice had smaller trophoblastic outgrowths than control embryos. Staging embryonic development at the time of flushing, however, revealed retarded embryo development in vivo in hormone-treated mice. After correlation with embryonic stage at the beginning of the culture, there was no difference in the size of trophoblastic outgrowths.
Treatment with gonadotropins impaired implantation and embryonic/fetal development. Changes in maternal milieu, rather than in embryo quality, may be responsible for the adverse effects observed.
Key wordsgonadotropins late implantation embryo/fetal development plasminogen activator mouse
Unable to display preview. Download preview PDF.
- 1.Ertzeid G, Storeng R: Adverse effects of gonadotropin treatment on pre- and postimplantation development in mice. J Reprod Fert 1992;96:649Google Scholar
- 2.Doyle P, Beral V, Maconochie: Preterm delivery, low birthweight and small-for-gestational-age in liveborn singleton babies resulting from in-vitro fertilization. Hum Reprod 1992;3:425Google Scholar
- 4.Elbling L, Colot M: Persistence of SCE-inducing damage in mouse embryos and fetuses following superovulation. Mutat Res 1989;76:117Google Scholar
- 5.Fossum GF, Davidson A, Paulson RJ: Ovarian hyperstimulation inhibits embryo implantation in the mouse. J Vitro Fert Embryo Transfer 1989;6:7Google Scholar
- 7.McRae AC, Church RB: Cytoplasmic projections of trophectoderm distinguish implanting from preimplanting and implantation-delayed mouse blastocysts. J Reprod Fert 1990;88:31Google Scholar
- 9.Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonald S, Pulford KAF, Stein H, Mason DY: Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 1984;32:212Google Scholar
- 10.Wilson ED, Edwards RG: Parturition and increased litter size in mice after superovulation. J Reprod Fert 1963;5:179Google Scholar
- 16.Pollard JW: Regulation of polypeptide growth factor synthesis and growth factor-related gene expression in the rat and mouse uterus before and after implantation. J Reprod Fert 1990;88:721Google Scholar