Abstract
Retinal adhesiveness and subretinal fluid absorption was studied in Dutch rabbit eyes given intravitreal injections of hemicholinium-3 (HC-3) which causes loss of photoreceptor outer segments and retinal pigment epithelial (RPE) damage. After HC-3 administration, some areas of the fundus showed pigmentary changes and others appeared normal. Small, non-rhegmatogenous retinal detachments were made in both areas. Within 2–5 days after HC-3 injection, only in the areas of visible damage, subretinal fluid spread laterally to make very flat retinal detachments, and the fluid absorbed very quickly. At later intervals, absorption was slower than normal, presumably because of scarring and RPE metabolic damage. HC-3 provides an experimental technique for transiently weakening retinal adhesiveness in vivo but its use as a model must account for the effects of both outer segment and RPE damage.
Similar content being viewed by others
References
Marmor MF. Mechanisms of normal retinal adhesion. In Ryan SJ, Schachat AP, Murphy RB, Patz A, eds. Retina. St. Louis: CV Mosby, 1989: 71–89.
Johnson LV, Hageman GS, Blancks JC. Interphotoreceptor matrix domains ensheath vertebrate cone photoreceptor cells. Invest Ophthalmol Vis Sci 1986; 27: 129–35.
Hollyfield JG, Varner HH, Rayborn ME, Osterfeld AM. Retinal attachment to the pigment epithelium. Retina 1989; 9: 59–68.
Yao X-Y, Hageman GS, Marmor MF. Retinal adhesiveness is weakened by enzymatic modification of the interphotoreceptor matrix in vivo. Invest Ophthalmol Vis Sci 1990; 31: 2051–58.
Marmor MF, Abdul-Rahim AS, Cohen DS. The effect of metabolic inhibitors on retinal adhesion and subretinal fluid resorption. Invest Opthalmol Vis Sci 1980; 19: 893–903.
Frambach DA, Marmor MF. The rate and route of fluid resorption from the subretinal space of the rabbit. Invest Ophthalmol Vis Sci 1982; 22: 292–302.
Negi A, Marmor MF. Mechanisms of subretinal fluid resorption in the cat eye. Invest Ophthalmol Vis Sci 1986; 27: 1560–63.
Pu GA-W, Masland PH. Biochemical interruption of membrane phospholipid renewal in retinal photoreceptor cells. J. Neurosci 1984; 4: 1559.
White MP, Negi A, Hock PA, Jain M, Marmor MF. Anatomical and functional effects of hemicholinium on the retina of Dutch rabbits. Invest Ophthalmol Vis Sci 1985; 26 (Suppl): 65.
White MP, Negi A, Hock PA. Effects of hemicholinium-3 on pigmented rabbit retina and pigment epithelium. Curr Eye Res 1990; 9; 669–76.
Marmor MF, Porteus M, Negi A, Immel J. Validation of a model of non-rhegmatogenous retinal detachments. Curr Eye Res 1984; 3: 515–18.
Negi A, Marmor MF. The resorption of subretinal fluid after diffuse damage to the retinal pigment epithelium. Invest Ophthalmol Vis Sci 1983; 24: 1475–79.
Negi A, Marmor MF. Experimental serious retinal detachment and focal pigment epithelial damage. Arch Ophthalmol 1984; 102: 445–49.
Yoon YH, Marmor MF. Effects of hemicholinium-3 and sodium iodate on RPE and retinal adhesiveness. Invest Ophthalmol Vis Sci 1991; 32 (Suppl): 667.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Negi, A., White, M.P. & Marmor, M.F. Effects of hemicholinium-3, a photoreceptor and pigment epithelial toxin, on retinal adhesiveness and subretinal fluid absorption. Doc Ophthalmol 83, 331–336 (1993). https://doi.org/10.1007/BF01204335
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01204335