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A GC/MS method for the analysis of 5-methyl-2′-deoxycytidine in DNA

Eine GC/MS Methode zur Analyse von 5-Methyl-desoxycytidin in DNA

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  • Biological and clinical analysis
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Summary

A method for the quantitative analysis of 5-methyl-2′-deoxycytidine (5mdC) in DNA based on enzymatic hydrolysis to nucleosides, separation and quantitation by GC/MS with internal standard has been developed. The method was applied to DNA from calf thymus and drosophila; the DNA from the Phage ΦX174 has been used to establish the accuracy of the procedure. The detection limit is 17 fmol 5mdC absolute and the content of modified cytosine in the DNA ofDrosophila melanogaster was shown to below that limit. Consequently, the genome contains less than one methylated cytidine per 330000 unmodified nucleosides or not more than 240 5mdC's overall, which renders an important function in regulation rather unlikely.

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Author notes

  1. C. Randt

    Present address: BASF AG, Ludwigshafen, Germany

Authors and Affiliations

  1. ISAS, Institut für Spektrochemie, Bunsen -Kirchhoff-Strasse 11, D-4600, Dortmund 1, Germany

    C. Randt & M. Linscheid

Authors
  1. C. Randt
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  2. M. Linscheid
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Dedicated to Prof. Dr. G. Tölg on the occasion of his 60th birthday

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Randt, C., Linscheid, M. A GC/MS method for the analysis of 5-methyl-2′-deoxycytidine in DNA. Z. Anal. Chem. 335, 865–868 (1989). https://doi.org/10.1007/BF01204103

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  • DOI: https://doi.org/10.1007/BF01204103

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