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Gastric carcinogenesis in rats given hypertonic salt at different times before a single dose ofN-methyl-N′-nitro-N-nitrosoguanidine

  • Original Papers
  • Experimental Oncology
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Abstract

A 1-ml dose of 4.5 M NaCl was given intragastrically to male Wistar rats at 10 min, 1 h, 4 h, 12 h, 24 h or 48 h before a single intragastric dose of 250 mg/kgN-methyl-N′-nitro-N-nitrosoguanidine (MNNG). After 52 weeks the incidence of forestomach squamous cell carcinoma was 21% in control animals receiving MNNG alone. The cancer incidence in the forestomach varied with the time elapsed between application of NaCl and MNNG, and was significantly increased in animals pretreated with NaCl 4 h (43%), 12 h (54%) and 24 h (41%) before MNNG. These results show that salt has a cocarcinogenic effect on initiation of forestomach carcinogenesis in rats, and that this effect depends on the time interval between pretreatment with NaCl and application of MNNG. Gastric adenocarcinomas occurred more frequently in the antrum (78%) than in the corpus (22%). The incidence of gastric adenocarcinoma in animals pretreated with salt before application of MNNG (11%–22%) was not significantly influenced by the time elapsed between pretreatment with salt and application of MNNG, and did not differ from animals receiving MNNG alone (18%). The lack of a cocarcinogenic effect of NaCl on glandular gastric carcinogenesis might be due to the use of dimethyl/sulfoxide as solvent for MNNG.

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Abbreviations

MNNG:

N-methyl-N′-nitro-N-nitrosoguanidine

DMSO:

dimethylsulfoxide

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Authors and Affiliations

  1. Surgical Research Laboratory, Department of Surgery, University of Bergen, Haukeland Hospital, N-5021, Bergen, Norway

    Halfdan Sørbye & Knut Svanes

  2. Department of Pathology, University of Bergen, Bergen, Norway

    Helga Maartmann-Moe

Authors
  1. Halfdan Sørbye
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  2. Helga Maartmann-Moe
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  3. Knut Svanes
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Additional information

This work was supported by grants from the Norwegian Cancer Society. Dr Sørbye is a Research Fellow of the Norwegian Cancer Society

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Sørbye, H., Maartmann-Moe, H. & Svanes, K. Gastric carcinogenesis in rats given hypertonic salt at different times before a single dose ofN-methyl-N′-nitro-N-nitrosoguanidine. J Cancer Res Clin Oncol 120, 159–163 (1994). https://doi.org/10.1007/BF01202195

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  • DOI: https://doi.org/10.1007/BF01202195

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