Abstract
Treatment with the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) and the second-stage promotor 12-O-retinoylphorbol 13-acetate induced down-regulation of protein kinase C (PKC) and enhanced the migration of C3H 10T1/2 cells. In the case of BT5C glioma cells the same negative correlation was observed only after treatment with TPA. The negative control 4α-phorbol affected neither PKC activity nor the migratory ability of both cell lines.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- PKC:
-
protein kinase C
- TPA:
-
12-O-tetradecanoylphorbol 13-acetate
- RPA:
-
12-O-retinoylphorbol
References
Bertram JS (1977) Effects of serum concentration on the expression of carcinogen-induced transformation in the C3H10T1/2 C18 cell line. Cancer Res 37:514–523
Bradford MM (1976) A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle opf protein-dye binding. Anal Biochem 72:248–254
Castagna M, Takai Y, Kaibuchi K, Sano K, Kikkawa U, Nishizuka Y (1982) Direct activation of calcium activated phospholipid dependent protein kinase by tumor promoting phorbol ester. J Biol Chem 257:7847–7851
Chida K, Hashida H, Sasaki K, Kuroki T (1986) Activation of protein kinase C and specific phosphorylation of a Mr 90.000 membrane protein of promotable BALB/3T3 and C3H/10T1/2 cells by tumor promoters. Cancer Res. 46:1055–1062
Droms KA, Malkinson AM (1991) Phorbol ester-induced tumor promotion by downregulation of protein kinase C. Mol Carcinog 4:1–2
Dugina VB, Svitkina TM, Vasiliev JM, Gelfand LM (1987) Special type of morphological reorganization induced by phorbol ester: reversible partition of cell into motile and stable domains. Proc Natl Acad Sci USA 84:4112–4125
Gschwendt M, Horn F, Kittstein W, Fürstenberger G, Marks F (1983) Soluble phorbol ester binding sites and phospholipid- and calcium-dependent protein kinase activity in cytosol of chick oviduct. FEBS Lett 162:147–150
Hansen LA, Monteiro-Riviere NA, Smart RC (1990) Differential down regulation of epidermal protein kinase C by 12-O-tetradecanoylphorbol-13-acetate and diacylglycerol: association with epidermal hyperplasia and tumor promotion. Cancer Res 50:5740–5745
Hergenhahn M, Kloz U, Fellhauer M, Tremp GL, Hecker E (1991) Toxicodynamics of tumour promoters of mouse skin. II. Binding to protein kinase C of some new diterpene esters and induction of luminolenhanced chemoluminescence in mouse peritoneal neutrophils. J Cancer Res Clin Oncol 117:385–395
Janik P, Szaniawska B, Miloszewska J, Pietruszewska E, Kowalczyk D (1992) The role of protein kinase C in migration of rat glioma cells from spheroid cultures. Cancer Lett 63:167–170
Kennedy AR, Little JB (1980) Investigation of the mechanism for enhancement of radiation transformation in vitro by 12-O-tetradecanoylphorbol-13-acetate. Carcinogenesis 1:1039–1047
Kraft AS, Anderson WB (1983) Phorbol esters increase the amount of Ca, phospholipid-dependent protein kinase associated with plasma membrane. Nature 301:621–623
Laerum OD, Rajewsky MF (1975) Neoplastic transformation of fetal rat brain cells in culture after exposure to ethylnitrosourea in vivo. J Natl Cancer Inst 55:1177–1187
Miloszewska J, Trawicki W, Janik P, Moraczewski J, Przybyszewska M, Szaniawska B (1986) Protein kinase C translocation in relation to proliferative status of C3H 10T1/2 cells. FEBS Lett 206:283–286
Mordan LJ, Bergin LM, Budnick JEL, Meegan RR, Bertram JS (1982) Isolation of methylcholanthrene-“initiated” C3H 10T1/2 cells by inhibiting neoplastic progression with retinyl acetate. Carcinogenesis 3:279–285
Nishizuka Y (1984) The role of protein kinase C in cell surface signal transduction and tumor promotion. Nature 308:693–698
Reznikoff CA, Brankow DW, Heidelberger C (1973a) Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to post confluence inhibition of division. Cancer Res 33:3231–3238
Reznikoff CA, Bertram JS, Brankow DW, Heidelberger C (1973b) Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res 33:3239–3249
Rodriguez-Pena A, Rozengurt E (1984) Disappearance of Ca-sensitive, phospholipid-dependent protein kinase activity in phorbol-ester treated 3T3 cells. Biochem Biophys Res Commun 120:1053–1059
Rosen EM, Jaken S, Carley W, Lucket PM, Setter E, Bhargava M, Goldberg JD (1991) Regulation of motility in bovine brain endothelial cells. J Cell Physiol 146:325–335
Schwartz GK, Redwood SM, Ohnuma T, Holland JF, Droller MJ, Liu BC-S (1990) Inhibition of invasion of invasivehuman bladder carcinoma cells by protein kinase inhibitor staurosporine. J Natl Cancer Inst 8:1753–1757
Schwartz GK, Jiang J, Kelsen D, Albino AP (1993) Protein Kinase C: a novel target for inhibiting gastric cancer cell invasion. J Natl Cancer Inst 85:402–407
Szaniawska B, Bjerkvig R, Laerum OD, Undheim K (1986) Growth arrest and polyploidization induced by metahalone microtubule inhibitors on rat glioma cells in culture. Anticancer Res 6:71–77
Yuhas JM, Li AP, Martinez AO, Ladman AJ (1977) A simplified method for production and growth of multicellular tumor spheroids. Cancer Res 37:3639–3643
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Janik, P., Szaniawska, B., Kowalczyk, D. et al. The effect of phorbol ester treatment on migration of C3H 10T1/2 and BT5C glioma cells: possible application to carcinogenesis. J Cancer Res Clin Oncol 120, 156–158 (1994). https://doi.org/10.1007/BF01202194
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01202194