Summary
Flow Injection Analysis of Trace Hydrogen Peroxide Using an Immobilized Enzyme Reactor
Sub-part-per-billion levels of aqueous hydrogen peroxide have been determined with a flow injection analysis system employing a single bead string reactor composed of horseradish peroxidase covalently bound to an animated macroporous polymeric adsorbent with glutaraldehyde and a passive cation exchange membrane reactor to alter pH. The chemistry relies on the peroxidase mediated oxidation of nonfluorescentp-hydroxyphenylacetate to its fluorescent dimer. The advantage of the system includes rapid throughout rates (40 samples/h), excellent detection limits (0.3 ppb H2O2) and large dynamic range of linear response (1 ppb-1 ppm). However, the immobilized enzyme is not useful for the analysis of organic peroxides which act as inhibitors.
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Hwang, H., Dasgupta, P.K. Flow injection analysis of trace hydrogen peroxide using an immobilized enzyme reactor. Mikrochim Acta 87, 77–87 (1985). https://doi.org/10.1007/BF01201987
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DOI: https://doi.org/10.1007/BF01201987