Abstract
Protoplasts ofCunninghamella elegans, showing 11α-, and 11β-hydroxylating ability of Substance S, preserved high transformation activity when dispersed in glucose-enriched, organic osmotic stabilizers. A joint action of polyoxins and 2-deoxy-d-glucose was necessary to prevent regeneration of the cell wall in long-lasting experiments. Stabilized and active, dispersed protoplasts may be an alternative research model for studying the function of the cell wall and intracellular metabolic pool constituents in steroid hydroxylation.
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Clark, T.A., Chong, R. & Maddox, I.S. 1982 The effect of dissolved oxygen tension on 11β-, and 19-hydroxylation of Reichstein's Substance S byPellicularia filamentosa.European Journal of Applied Microbiology and Biotechnology 14, 131–135
Clark, T.A., Maddox, I.S. & Chong, R. 1983 The effect of glucose on 11β-, and 19-hydroxylation of Reichstein's Substance S byPellicularia filamentosa.European Journal of Applied Microbiology and Biotechnology 17, 211–215.
Dŀugoński, J., Bartnicka, K., Zemeŀko, I., Chojecka, V. & Sedlaczek, L. 1991 Determination of cytochrome P-450 inCunninghamella elegans intact protoplasts and cell-free preparations capable of steroid hydroxylation.Journal of Basic Microbiology 31, 347–356.
Dŀugoński, J. & Sedlaczek, L. 1988 Immobilization of fungal protoplasts for steroid bioconversion.Acta Microbiologica Polonica 37, 53–60.
Dŀugoński, J., Sedlaczek, L. & Jaworski, A. 1984 Protoplast release from fungi capable of steroid transformation.Canadian Journal of Microbiology 30, 57–62.
Hanisch, W.H., Dunhill, P. & Lilly, M.D. 1980 Optimization of the production of progesterone 11α-hydroxylase byRhizopus nigricans.Biotechnology and Bioengineering 22, 555–570.
Jaworski, A., Sedlaczek, L. & Dŀugoński, J. 1984 Transformation of steroids by fungal spores. III. Activity of the 11-hydroxylases at various times during germination and vegetative growth ofCunninghamella elegans sporangiospores.Applied Microbiology and Biotechnology 20, 313–317.
Kastelic-Sahadolc, T. & Belič, I. 1991 Involvement of the cell wall ofNocardia restricta on the bioconversions of steroid sapogenins.World Journal of Microbiology & Biotechnology 7, 22–24.
Komel, R., Rozman, D., Puc, A. & Sošič, H. 1985 Effect of immobilization on the stability ofClaviceps purpurea protoplasts.Applied Microbiology and Biotechnology 23, 106–109.
Makins, J.F., Holt, G. & Macdonald, K.D. 1980 Co-synthesis of penicillin following treatment of mutants ofAspergillus nidulans impaired in antibiotic production with lytic enzymes.Journal of General Microbiology 119, 397–404.
Sedlaczek, L. 1988 Biotransformation of steroids.Critical Reviews in Biotechnology 7, 187–236.
Sedlaczek, L., Dŀugoński, J. & Jaworski, A. 1984 Transformation of steroids by fungal protoplasts.Applied Microbiology and Biotechnology 20, 166–169.
Sedlaczek, L., Jaworski, A. & Wilmańska, D. 1981 Transformation of steroids by fungal spores. I. Chemical changes ofCunninghamella elegans spores and mycelium during cortexolone hydroxylation.European Journal of Applied Microbiology and Biotechnology 13, 155–160.
Žakelj-Mavrič, M., Plemenitas, A., Komel, R. & Belič, I. 1990 11β-Hydroxylation of steroids byCochliobolus lunatus.Journal of Steroid Biochemistry 35, 627–629.
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Dtugoński, J., Bartnicka, K., Chojecka, V. et al. Stabilization of steroid 11-hydroxylation activity ofCunninghamella elegans protoplasts in organic osmotic stabilizers. World J Microbiol Biotechnol 8, 500–504 (1992). https://doi.org/10.1007/BF01201948
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DOI: https://doi.org/10.1007/BF01201948