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Mould phytases and their application in the food industry

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Abstract

The nutritional implications of the presence of phytates in certain food products are described. Several techniques for phytate removal or conversion in foods are discussed, with special emphasis on enzymic conversion. The application of mould phytases for the dephosphorylation of phytate compounds is evaluated with respect to legume products, breadmaking, chicken diets and corn wet milling. The properties of different phytases are summarized and data concerning biosynthesis of phytate-degrading enzymes are also analysed.

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References

  • Anon. 1989 ‘Natuphos’ keeping up with nature. Gist-brocades contributes to solving the problem of phosphate pollution related to animal manure. Koninklijke Gist-brocades NV, Agro Business Group.

  • Beal, L. & Mehta, T. 1985 zinc and phytate distribution in peas. Influence of heat treatment, germination, pH, substrate, and phosphorus on pea phytate and phytase.Journal of Food Science 50, 96–100, 115.

    Google Scholar 

  • Brooks, J.R. & Morr, C.W. 1982 Phytate removal from soy protein isolates using ion exchange processing treatments.Journal of Food Science 47, 1280–1282.

    Google Scholar 

  • Caransa, A., Simell, M., Lehmussaari, M., Vaara, M. & Vaara, T. 1988. A novel enzyme application in corn wet milling.Starch 40, 409–411.

    Google Scholar 

  • Chang, R., Schwimmer, S. & Burr, H.K. (1977) Phytate: removal from whole dry beans by enzymatic hydrolysis and diffusion.Journal of Food Science 42, 1098–1101.

    Google Scholar 

  • Chompreeda, P.T. & Fields, M.L. 1984 Effects of heat and fermentation on the extractability of minerals from soybean meal and corn meal blends.Journal of Food Science 49, 566–568.

    Google Scholar 

  • Clydesdale, F.M. & Camire, A.L. 1983 Effect of pH and heat on the binding of iron, calcium, magnesium, and zinc and the loss of phytic acid in soy flour.Journal of Food Science 48, 1272–1274, 1283.

    Google Scholar 

  • Cooper, J.R. & Gowing, H.S. 1983 Mammalian small intestinal phytase (EC 3.1.3.8).British Journal of Nutrition 50, 673–678.

    Google Scholar 

  • De Boland, A.R., Garner, G.B. & O'Dell, B.L. 1975 Identification and properties of “phytate” in cereal grains and oilseed products.Journal of Agricultural and Food Chemistry 23, 1186–1189.

    Google Scholar 

  • Deshpande, S.S. & Cheryan, M. 1984 Effect of phytic acid, divalent cations, and their interaction on α-amylase activity.Journal of Food Science 49, 516–519.

    Google Scholar 

  • Deshpande, S.S. & Damodaran, S. 1989 Effect of phytate on solubility, activity and conformation of trypsin and chymotrypsin.Journal of Food Science 54, 695–699.

    Google Scholar 

  • Empson, K.L., Labuza, T.P. & Graf, E. 1991 Phytic acid as a food antioxidant.Journal of Food Science 56, 560–563.

    Google Scholar 

  • Eskin, N.A.M. & Wiebe, S. 1983 Changes in phytase activity and phytate during germination of two fababean cultivars.Journal of Food Science 48, 270–271.

    Google Scholar 

  • Faridi, H.A., Finney, P.L. & Rubenthaler, G.L. 1983 Effect of soda leavening on phytic acid content and physical characteristics of Middle Eastern breads.Journal of Food Science 48, 1654–1658.

    Google Scholar 

  • Ferrel, R.E. 1978 Distribution of bean and wheat inositol phosphate esters during autolysis and germination.Journal of Food Science 43, 563–565.

    Google Scholar 

  • Fretzdorff, B. 1989 Phytinsäure in Weizenkleien und Weizenkeimen Möglichkeiten zur Entfernung der Phytinsäure.Zeitschrift für Lebensmiitteln Untersuchung und Forschung 189, 110–112.

    Google Scholar 

  • Gibson, D.M. 1987 Production of extracellular phytase fromAspergillus ficuum on starch media.Biotechnology Letters 5, 305–310.

    Google Scholar 

  • Gifford, S.R. & Clydesdale, F.M. 1990 Interactions among calcium, zinc and phytate with three protein sources.Journal of Food Science 55, 1720–1723.

    Google Scholar 

  • Greaves, M.P., Anderson, G. & Webley, D.M. 1967 The hydrolysis of inositol phosphate byAerobacter aerogenes.Biochimica et Biophysica Acta 132, 412–418.

    Google Scholar 

  • Han, Y.W. 1988 Removal of phytic acid from soybean and cotton seed meals byAspergillus ficuum phytase.Journal of Agricultural and Food Chemistry 36, 1181–1183.

    Google Scholar 

  • Han, Y.W. & Gallagher, D.J. 1987 Phosphatase production byAspergillus ficuum.Journal of Industrial Microbiology 1, 377–382.

    Google Scholar 

  • Han, Y.W., Gallagher, D.J. & Wilfred, D.J. 1987 Phytase production byAspergillus ficuum on semi-solid substrate.Journal of Industrial Microbiology 2, 195–200.

    Google Scholar 

  • Han, Y.W. & Wilfred, D.J. 1988 Phytate hydrolysis in soybean and cottonseed meals byAspergillus ficuum phytase.Journal of Agricultural and Food Chemistry 36, 259–262.

    Google Scholar 

  • Hara, A., Ebina, S., Kondo, A. & Funaguma, T. 1985 A new type of phytase from pollen ofTypha latifolia L.Agricultural and Biological Chemistry 49, 3539–3544.

    Google Scholar 

  • Hayakawa, T., Okada, F., Tsutsui, M., Sato, N. & Igaue, I. 1991 Effect of phytate on the hydrolysis of p-nitrophenyl phosphate with phosphatase activity from various sources.Agricultural and Biological Chemistry 55, 651–657.

    Google Scholar 

  • Hayakawa, T., Suzuki, K., Miura, H., Ohno, T. & Igaue, I. 1990 Myo-inositol polyphosphate intermediates in the dephosphorylation of phytic acid by acid phosphatase with phytase activity from rice bran.Agricultural and Biological Chemistry 54, 279–286.

    Google Scholar 

  • Hayakawa, T., Toma, Y. & Igaue, I. 1989 Purification and characterization of acid phosphatases with or without phytase activity from rice bran.Agricultural and Biological Chemistry 53, 1475–1483.

    Google Scholar 

  • Henderson, H.M. & Ankrah, S.A. 1985 The relationship of endogenous phytase, phytic acid and moisture uptake with cooking time inVicia faba minor cv. Aladin.Food Chemistry 17 1–11.

    Google Scholar 

  • Howson, S.J. & Davis, R.P. 1983 Production of phytate-hydrolysing enzyme by some fungi.Enzyme and Microbial Technology 5, 377–382.

    Google Scholar 

  • Irving, G.C. & Cosgrove, D.J. 1974 Inositol phosphate phosphatases of microbial origin. Some properties of the partially purified phosphatases ofAspergillus ficuum NRRL 3135.Australian Journal of Biological Sciences 27, 361–368.

    Google Scholar 

  • Khan, N., Zaman, R. & Elahi, M. 1988 Effect of processing on the phytic acid content of bengal grams (Cicer arietinum) products.Journal of Agricultural and Food Chemistry 36, 1274–1276.

    Google Scholar 

  • Khetarpaul, N. & Chauhan, B.M. 1990 Effects of germination and pure culture fermentation by yeasts and lactobacilli on phytic acid and polyphenol content of pearl millet.Journal of Food Science 55, 1180–1181.

    Google Scholar 

  • Kiiskinen, T. & Piironen, J. 1990 Effect of phytase supplementation on utilization of phosphorus in chicken diets. InProceedings of 8th European Poultry Conference, pp. 376–380. Barcelona: World Poultry Science Association, Fira de Barcelona.

    Google Scholar 

  • Knorr, D., Watkins, T.R. & Carlson, B.L. 1981 Enzymatic reduction of phytate in whole wheat breads.Journal of Food Science 46, 1866–1869.

    Google Scholar 

  • Knuckles, B.E., Kuzmicky, D.D., Gumbmann, M.R. & Betschart, A.A. 1989 Effect of myo-inositol phosphate esters onin vitro andin vivo digestion of protein.Journal of Food Science 54, 1348–1350.

    Google Scholar 

  • Kratzer, F.H. & Vohra, P. (eds) 1986 Role of phytic acid and other phosphates as chelating agents. InChelates in Nutrition, pp. 49–61. Boca Raton, FL: CRC Press.

    Google Scholar 

  • Lim, P.E. & Tate, M.E. 1973 The phytases. II. Properties of phytase fraction F1 and F2 from wheat bran and the myo-inositol phosphates produced by fraction F2.Biochimica et Biophysica Acta 302, 316–328.

    Google Scholar 

  • Lopez, Y., Gordon, D.T. & Fields, M.L. 1983 Release of phosphorus from phytate by natural lactic acid fermentation.Journal of Food Science 48, 953–954.

    Google Scholar 

  • Martin, M. & Luque, J. 1985 Phytase activity in chicken erythrocytes and its control by organic phosphates (glycerate 2,3-P2 and inositol P5) during avian development.Comparative Biochemistry and Physiology 80 B, 557–561.

    Google Scholar 

  • Nair, V.C. & Duvnjak, Z. 1991 Phytic acid content reduction in canola meal by various microorganisms in a solid state fermentation process.Acta Biotechnologica 11, 211–218.

    Google Scholar 

  • Nayini, N.R. & Markakis, P. 1983 Effect of fermentation time on the inositol phosphates of bread.Journal of Food Science 48, 262–263.

    Google Scholar 

  • Nayini, N.R. & Markakis, P. 1984 The phytase of yeast.Lebensmittel—Wissenschaft und Technologie 17, 24–26.

    Google Scholar 

  • Nelson, T.S. 1976 The hydrolysis of phytate phosphorus by chicks and laying hens.Poultry Science 55, 2262–2264.

    Google Scholar 

  • Nelson, T.S., Shieh, T.R., Wodzinski, R.J. & Ware, J.H. 1971 Effect of supplement phytase on the utilization of phytate phosphorus by chicksJournal of Nutrition 101, 1289–1294.

    Google Scholar 

  • Nyman, M.E. & Björck, I.M. 1989 In vivo effects of phytic acid and polyphenols on the bioavailability of polysaccharides and other nutrients.Journal of Food Science 54, 1332–1335.

    Google Scholar 

  • Pears, F.G. 1953 The phytase of wheat.Biochemical Journal 53, 102–109.

    Google Scholar 

  • Phillippy, B.Q., White, K.D., Johnston, M.R., Tao, S.-H. & Fox, M.R.S. 1987 Preparation of inositol phosphates from sodium phytate by enzymatic and nonenzymatic hydrolysis.Analytical Biochemistry 162, 115–121.

    Google Scholar 

  • Platt, S.R. & Clydesdale, F.M. 1987 Interactions of iron, alone and in combination with calcium, zinc and copper, with phytate-rich, fibre-rich fraction of wheat bran under gastrointestinal pH conditions.Cereal Chemistry 64, 102–105.

    Google Scholar 

  • Raport, S. & Guest, G.M. 1941 Distribution of acid-soluble phosphorus in blood cells of various vertebrates.Journal of Biological Chemistry 138, 269–282.

    Google Scholar 

  • Reichelt, J.R. 1983 Industrial applications. Baking. InIndustrial Enzymology. The Applications of Enzymes in Industry eds Godfrey, T. & Reichelt, J.R. pp. 210–220. New York: The Nature Press.

    Google Scholar 

  • Scott, J.J. & Loewus, F.A. 1986 A calcium activated phytase from pollen ofLilium longiflorum.Plant Physiology 82, 333–335.

    Google Scholar 

  • Serraino, M.R. & Thompson, L.U. 1984 Removal of phytic acid and protein-phytic acid interactions in rapeseed.Journal of Agricultural and Food Chemistry 32, 38–40.

    Google Scholar 

  • Shieh, T.R., Wodzinski, R.J. & Ware, J.H. 1969 Regulation of the formation of acid phosphatases by inorganic phosphate inAspergillus ficuum.Journal of Bacteriology 100, 1161–1165.

    Google Scholar 

  • Singh, B. & Sedeh, H.G. 1979 Characteristics of phytase and its relationship to acid phosphatase and certain minerals in triticale.Cereal Chemistry 56, 267–272.

    Google Scholar 

  • Skowronski, T. 1978 Some properties of partially purified phytase fromAspergillus niger.Acta Microbiologica Polonica 27, 41–48.

    Google Scholar 

  • Sudarmadji, S. & Markakis, P. 1977 The phytate and phytase of soybean tempeh.Journal of the Science of Food and Agriculture 28, 381–383.

    Google Scholar 

  • Sutardi, & Buckle, K.A. 1985 Phytic acid changes in soybeans fermented by traditional inoculum and six strains ofRhizopus oligosporus.Journal of Applied Bacteriology 58, 539–543.

    Google Scholar 

  • Sutardi, & Buckle, K.A. 1986 The characteristics of soybean phytase.Journal of Food Biochemistry 10, 197–216.

    Google Scholar 

  • Sutardi, & Buckle, K.A. 1988 Characterization of extra- and intracellular phytases fromRhizopus oligosporus used in tempeh production.Journal of Food Microbiology 6, 67–79.

    Google Scholar 

  • Tangkongchitr, U., Seib, P.A. & Hoseney, R.C. 1981 Phytic acid. Its fate during breadmaking.Cereal Chemistry 58, 229–234.

    Google Scholar 

  • Tangkongchitr, U., Seib, P.A. & Hoseney, R.C. 1982 Phytic acid. III. Two barriers to the loss of phytate during breadmaking.Cereal Chemistry 59, 216–221.

    Google Scholar 

  • Wang, H.L., Swain, E.W. & Hesseltine, C.W. 1980 Phytase of molds used in oriental food fermentation.Journal of Food Science 45, 1262–1266.

    Google Scholar 

  • Yamada, K., Minoda, Y. & Yamamoto, S. 1968 Phytase fromAspergillus tereus. Part I. Production, purification and some general properties of the enzyme.Agricultural and Biological Chemistry 32, 1275–1282.

    Google Scholar 

  • Yoon, J.H., Thompson, L.U. & Jenkins, D.J.A. 1983 The effect of phytic acid onin vitro rate of starch digestibility and blood glucose response.American Journal of Clinical Nutrition 38, 835–842.

    Google Scholar 

  • Zemel, M.B. & Shelf, L.A. 1982 Phytic acid hydrolysis and soluble zinc and iron in whole wheat bread as affected by calcium containing additives.Journal of Food Science 47, 535–537.

    Google Scholar 

  • Żyła, K. 1991 Products of enzymic dephosphorylation of phytate determined by a simple colorimetric method.Biotechnology Techniques 5, 127–132.

    Google Scholar 

  • Żyła, K., Koreleski, J. & Kujawski, M. 1989 Dephosphorylation of phytate compounds by means of acid phosphatase fromAspergillus niger.Journal of the Science of Food and Agriculture 49, 315–324.

    Google Scholar 

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  1. Department of Food Biotechnology, University of Agriculture, 29-Listopada Ave., 46, 31-425, Krakow, Poland

    K. Żyta

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Żyta, K. Mould phytases and their application in the food industry. World J Microbiol Biotechnol 8, 467–472 (1992). https://doi.org/10.1007/BF01201941

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