Abstract
Introduced clovers (Trifolium repens andTrifolium subterraneum) fall to persist more than two years in Uruguayan improved pastures. Three strains of naturalizedRhizobium leguminosarum bvtrifolii, isolated from persistent clover pastures inoculated several years ago, and two commercial inoculant strains have been identified. Serological techniques (enzyme-linked immunosorbent assay—ELISA) and total cell protein pattern have been selected as sensitive and reliable methods for strain identification. Indirect ELISA was suitable for detecting rhizobia in cultures or in nodules. Two of the naturalized strains had distinct protein profiles but they could not be differentiated by the immunological method with a polyclonal antibody.
Résumé
Des trèfies (Trifolium repens etTrifolium subterraneum) introduits dans des pâturages améliorés d'Uruguay, n'ont pu s'y maintenir plus de deux années. On a identifié 3 souches naturalisées deRhizobium leguminoserum bvtrifolii de pâturages aux trèfies persistants, inoculés il y a plusleurs années, ainsi que 2 souches d'inoculation industrielles. Des techniques sérologiques (ELISA: enzyme-linked immunosorbent assay) et l'histogramme des protéines cellulaires totales ont été sélectionnées comme méthodes sensibles et fiables pour l'identification des souches. Le test ELISA indirect est vaiable pour détecter les rhizobia en culture ou dans les nodules. Deux des souches naturalisées avaient leur propre profil protéique mais elles n'ont pu être différenclées par la méthode immunologique utilisant un anticorps polyclonal.
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Fabiano, E., Arias, A. Identification of inoculant strains and naturalized populations ofRhizobium leguminosarum bvtrifolii using complementary methodologies. World J Microbiol Biotechnol 6, 121–126 (1990). https://doi.org/10.1007/BF01200931
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DOI: https://doi.org/10.1007/BF01200931