Abstract
Glucose prevented maltose utilization in batch culture ofSaccharomyces cerevisiae whereas in a mixed carbohydrate-limited system, maltose and glucose were consumed simultaneously. The specific activity of α-glucosidase depended on the dilution rate as well as the proportion of maltose in the mixture. The chemostat provides a way of reaching the low residual concentrations of glucose in the broth that are necessary to release catabolite repression and permit maltose induction of α-glucosidase.
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References
Federoff, H.J., Cohen, J.D., Eccleshall, T.R. et al. 1982 Isolation of a maltase structural gene fromSaccharomyces carlsbergensis.Journal of Bacteriology 149, 1064–1070.
Geeta, R., Rale, V.B. & Hegde, M.V. 1990 Decryptification ofS. carlsbergensis with dimethylsulfoxide for rapid determination of α-glucosidase.Indian Journal of Microbiology 30, 427–431.
Gorts, C.P.M. 1969 Effect of glucose on the activity and the kinetics of the maltose uptake system and of α-glucosidase inSaccharomyces cerevisiae.Biochimica et Biophysica Acta 184, 229–305.
Halvorson, H.O. & Ellias, L. 1958 The purification and properties of an α-glucosidase ofSaccharomyces italicus 1225.Biochimica et Biophysica Acta 184, 28–40.
Mortimer, R.K. & Schild, D. 1985 Genetic map ofSaccharomyces cerevisiae. Edition 9.Microbiological Reviews 49, 181–212.
Schaeffer, E.J. & Cooney, C.L. 1982 Production of maltase by Wild-Type and a constitutive mutant ofSaccharomyces italicus.Applied and Environmental Microbiology 43, 75–80.
Siro, M.R. & Lovgren, T. 1979 Influence of glucose on the α-glucosidase permease activity ofYeast.European Journal of Applied Microbiology and Biotechnology 7, 59–66.
Sollitti, P. & Marmur, J. 1988 Primary structure of the regulatory gene from theMAL 6 locus ofSaccharomyces carlsbergensis.Molecular and General Genetics 213, 56–62.
Van Rijn, J. & Van Wijk, R. 1972 Differential sensitivities of the two malate dehydrogenases and the maltose permease to the effect of glucose inSaccharomyces carlsbergensis.Journal of Bacteriology 110, 477–484.
Zimmermann, F.K. & Eaton, N.R. 1974 Genetics of induction and catabolite repression of maltase synthesis inSaccharomyces cerevisiae.Molecular and General Genetics 134, 261–272.
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Ramesh, G., Deshpande, M.S., Maggirwar, S.B. et al. α-Glucosidase synthesis in batch and continuous culture ofSaccharomyces cerevisiae . World J Microbiol Biotechnol 8, 42–44 (1992). https://doi.org/10.1007/BF01200682
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DOI: https://doi.org/10.1007/BF01200682