Summary
A flow injection system with a reactor containing peroxidase immobilized on porous glass was used for the determination of hydrogen peroxide. A number of chromogenic reagents were studied in combination with different immobilization methods, in particular with regard to adsorption of the coloured product on the enzyme support. A f. i. a. manifold had a throughout of 210 samples h−1 and a detection limit of 0.1 μM H2O2 and could be used for at least 17000 determinations with the same enzyme reactor. Systems with higher sensitivity or higher sample throughput are also described.
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References
L. C. Clark Jr., in: Methods in Enzymology, Vol. 56. New York: Academic Press 1979, p. 448.
T. E. Barman, Enzyme Handbook, Vol. 1, New York: Springer 1969.
H. B. Dunford and J. S. Stillman, Coord. Chem. Rev.19, 187 (1976).
T. T. Ngo and H. M. Lenhoff, Anal. Biochem.105, 389 (1980).
B. Olsson, L. Ögren, and G. Johansson, Anal. Chim. Acta145, 101 (1983).
K. Nilsson and P. O. Larsson, Anal. Biochem.134, 60 (1983).
B. Olsson and L. Ögren, Anal. Chim. Acta145, 87 (1983).
D. Barham and P. Trinder, Analyst97, 142 (1972).
K. Tamaoku, Y. Murao, K. Akiura, and Y. Ohkura, Anal. Chim. Acta136, 121 (1982).
P. Thibault, P. Monsan, and C. Jouret, Sci. Aliment.1, 55 (1981).
C. Flemming, A. Gabert, and H. Wand, Acta Biol. Med. Ger.32, 135 (1974).
Kikutake Junichiro, Jpn. Pat., Jpn. Kokai Tokyo Koho,78, 104, 789 (1978).
R. Epton, G. Marr, and R. G. Ridley, Polymer20, 1447 (1979).
R. A. Messing and H. H. Weetall, U. S. Pat.3, 519, 538 (1970).
F. E. Regnier and R. Noel, J. Chrom. Sci.14, 316 (1976).
W. E. Hornby and H. Filippusson, Biochem. J.120, 215 (1970).
G. P. Royer, F. A. Liberatore, and G. M. Green, Biochim. Biophys. Res. Commun.64, 478 (1975).
G. S. Bethell, J. S. Ayers, W. S. Hancock, and M. T. W. Hearn, J. Biol. Chem.254, 2572 (1979).
I. Weinryb, Biochim. Biophys. Res. Commun.31, 110 (1968).
J. H. Bovaird, T. T. Ngo, and H. M. Lenhoff, Clin. Chem.28, 2423 (1982).
G. Johansson, L. Ögren, and B. Olsson, Anal. Chim. Acta145, 71 (1983).
P. W. Carr and L. D. Bowers, Immobilized Enzymes in Analytical and Clinical Chemistry (Chemical Analysis, Vol. 56). New York: John Wiley & Sons 1980.
H. Lundbäck and B. Olsson, in manuscript.
W. J. Blaedel and J. M. Uhl, Clin. Chem.21, 119 (1975).
D. L. Witte, L. F. Brown, and R. D. Feld, Clin. Chem.24, 1778 (1978).
B. Olsson and H. Lundbäck, in manuscript.
R. H. White-Stevens and L. R. Stover, Clin. Chem.28, 589 (1982).
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Olsson, B. A flow-injection system using immobilized peroxidase and chromogenic reagents for possible determination of hydrogen peroxide. Mikrochim Acta 86, 211–221 (1985). https://doi.org/10.1007/BF01197821
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DOI: https://doi.org/10.1007/BF01197821