Summary
Two dimensional thin layer Chromatographic techniques have been used in the study of the carotenoid pigments in the seeds of Bixa orellana L. In addition to the two major carotenoids bixin and norbixin,β-carotene, cryptoxanthin, lutein, zeaxanthin and methyl bixin have been tentatively identified by co-chromatography. There were other unidentified carotenoids in trace amounts. The probable biosynthetic significance of these carotenoids in relation to bixin is discussed.
Zusammenfassung
Zweidimensionale Dünnschichtchromatographie wurde zur Untersuchung der Karotinoid-Farbstoffe in den Samen von Bixa orellana L. verwendet. Außer den beiden Hauptbestandteilen Bixin und Norbixin wurdenβ-Karotin, Kryptoxanthin, Lutein, Zeaxanthin und Methylbixin chromatographisch festgestellt. Außerdem fanden sich andere nicht identifizierte Karotinoide in Spuren. Die wahrscheinliche biosynthetische Bedeutung dieser Karotinoide im Hinblick auf Bixin wurde diskutiert.
Similar content being viewed by others
References
C. S. Byles, J. Jamaica Agr. Soc.40, 220 (1936).
H. H. Zeylstra, C. T. T. van Hall, and C. van de Koppel, De landbouw in de Indische Archipel III. The Hague: Van Hoeve. 1950. p. 565.
W. B. Mors and C. T. Rizzini, Useful Plants of Brazil, San Francisco: Holden-Day. 1966. p. 55.
L. Santamaria, J. Agr. Univ. Puerto Rico49, 259 (1965).
T. C. Moomaw and N. Matsumo, Techn. Progress. Rep. Hawaii Agr. Exp. Station129, 1 (1961).
M. R. Sanchez, Agricultura Trop.21, 224 (1965).
T. W. Goodwin in K. Peach and M. V. Tracey (eds.), Modern Methods of Plant Analysis, Vol. III. Berlin-Göttingen -Heidelberg: Springer-Verlag. 1955. p. 272.
McKeown and J. Mark, J. Assoc. Official Agric. Chem.44, 761 (1962).
R. M. C. Dawson, Daphne C. Elljott, W. H. Elliott, and K. M. Jones, in Data of Biochemical Research, 2nd Ed., Oxford: Clarendon Press. 1969. p. 513.
l. c. 9, p. 514.
E. Sartori and M. Bolletti, Experientia4, 270 (1948).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tirimanna, A.S.L. Study of the carotenoid pigments of Bixa orellana L. Seeds by thin layer chromatography. Mikrochim Acta 76, 11–16 (1981). https://doi.org/10.1007/BF01197299
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01197299