Summary
The resistance of melanin to acid attack is used in a new gravimetric procedure for the determination of melanin in animal tissues. Lipids are removed by solvent extraction after acid hydrolysis of the samples.
Zusammenfassung
Eine neue gravimetrische Methode zur Bestimmung von Melanin in tierischen Geweben wurde entwickelt, die auf dessen Widerstandsfähigkeit in saurem Milieu beruht. Die vorgeschlagene Methode ist einfach; ihre Resultate werden in absoluten Werten ohne Kalibrierung gewonnen. Sie läßt sich für verschiedene Pigmentgewebe anwenden. Das beschriebene Verfahren scheint vorteilhafter als visuelle Schätzungsmethoden und solche Verfahren zu sein, die eine Melaninauflösung vorsehen. Die Melaninkonzentration in einigen Pigmentgeweben wurde angegeben.
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Borovanský, J. Quantitative determination of melanin. Mikrochim Acta 70, 423–429 (1978). https://doi.org/10.1007/BF01197094
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DOI: https://doi.org/10.1007/BF01197094