Summary
The formation of fluorescamine derivatives of tyrosine, triiodothyronine (T3) and tetraiodothyronine (T4) has been studied. The three compounds react quantitatively within 60 sec and form strongly fluorescent derivatives with an emission maximum at 490 nm. Limits of detection are between 26 ng/ml (T3) and 50 ng/ml (T4), if the reaction mixture is measured directly. Excellent TLC-separations on cellulose are possible for the derivatives of T3 and T4. In-situ fluorescence measurements result in linear calibration curves up to 1μg (T4) with a limit of detection of 1.5 ng/spot.
Zusammenfassung
Die Herstellung von Fluorescamin-Derivaten des Tyrosins, des Trijodthyronins (T3) und des Tetrajodthyronins (T4) wurde untersucht. Diese drei Verbindungen reagieren quantitativ innerhalb 60 sec unter Bildung stark fluoreszierender Derivate mit einem Emissionsmaximum bei 490 nm. Die Nachweisgrenzen liegen zwischen 26 ng/ml (T3) und 50 ng/ml (T4), wenn man das Reaktionsgemisch direkt zur Messung verwendet. Die T3-und T4-Derivate lassen sich auf Zellulose-Dünnschichten sehr gut trennen. In-situ-Messungen der Fluoreszenz ergaben Eichgerade bis 1μgT 4 mit einer Nachweisgrenze von 1,5 ng/Fleck.
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This paper is dedicated to Prof. Dr. H. Lieb on the occasion of his 90th birthday.
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Reiterer, F., Nachtmann, F., Knapp, G. et al. Fluorimetric determination of tyrosine and iodinated thyronines as the fluorescamine derivatives. Mikrochim Acta 69, 115–124 (1978). https://doi.org/10.1007/BF01196986
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DOI: https://doi.org/10.1007/BF01196986