Abstract
A specific radioreceptor assay, for squamous-cell carcinoma of the human esophagus, using 2G3 monoclonal antibody, has been developed for the first time. In this assay a new mathematical parameter of the Scatchard plot has been introduced for the correct measure of binding capacity (BCcm=x cos α). This is the perpendicular distance from the origin to the Scatchard plot line. It is always a positive quantity and is directly proportional to both thex andy intercepts for expressing the binding capacity. The assay is highly sensitive and can be used to differentiate various types of esophageal tumors such as squamous-cell carcinoma, adenocarcinoma, ulcerative growth and also a benign growth. BCcm significantly varies in the case of squamous-cell carcinoma tumors compared to adenocarcinoma and other types of tumor of the human esophagus. The assay can be completed in about 4 h and it may be used as a clinical diagnostic test.
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References
Adcock MR, Kelly PA (1992) Identification of ligand binding determinants of the prolaction receptors. J Biol Chem 267:7428–7493
Bernal SD, Speak JA (1984) Membrane antigen in small cell carcinoma of the lung defined by monoclonal antibody SMI. Cancer Res 44:265–270
Hogeboom GH (1955) Fractionation of cell component of animal tissues. Methods Enzymol 1:16–19
Liberti PA, Hackett CJ, Askonas BA (1979) Influenza virus infection of mouse lymphoblasts alters the binding affinity of anti-H-2 antibody: requirement for viral neuraminidase. Eur J Immunol 9:751–757
Markwell MAK, Fox CF (1978) Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachoro-3α-6α-diphenylglycoluril. Biochemistry 17:4807–4817
Scatchard G (1949) The attraction of proteins for small molecules and ions. Ann NY Acad Sci 51660–672
Takao T, Mitchell WMM, Souza EB de (1991) Interleukin-1 receptors in mouse kidney: identification, localization and modulation by lipopolysaccaride treatment. Endocrinology 128:2618–2624
Waingankar PR, Nadkarni JS (1990) Novel monoclonal antibody for the diagnosis of carcinoma of the esophagus (abstract). Eur J Cancer 26:1015
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Panse, G.T., Nadkarni, J.S. & Nadkarni, J.J. A specific and rapid receptor assay for squamous-cell carcinoma of human esophagus. J Cancer Res Clin Oncol 119, 734–736 (1993). https://doi.org/10.1007/BF01195345
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DOI: https://doi.org/10.1007/BF01195345