Abstract
Female 6-week-old shrews were given a solution ofN-methyl-N′-nitroN-nitrosoguanidine (MNNG) at a concentration of 50 μg/ml or 100 μg/ml in the drinking water. All 11 shrews receiving 100 μg/ml MNNG died 8–13 days after the beginning of carcinogen administration and 6 of the 20 shrews receiving 50 μg/ml MNNG died after 10–54 days. When animals were between 43 and 54 weeks of age, multiple esophageal lesions were evoked in all 14 that had received 50 μg/ml MNNG for 30 weeks. All shrews developed a protruding, ulcerative, or superficial type of squamous-cell carcinoma of the esophagus, accompanied by papillomas. Local invasion was seen in squamous-cell carcinoma but no distant metastasis was noted. None of the 5 control shrews developed any esophageal abnormality. No gastric adenocarcinoma, intestinal sarcoma, or other tumors were induced with MNNG. It can be concluded that MNNG has a carcinogenic effect on shrew esophageal epithelium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- MNNG:
-
N-methyl-N′-nitro-N-nitrosoguanidine
- ENNG:
-
N-ethyl-N′-nitro-N-nitrosoguanidine
- BrdU:
-
5-bromo-2′-deoxyuridine
References
Adamson RH, Sieber SM (1983) Chemical carcinogenesis studies in nonhuman primates. In: Langenbach R, Nesnow S, Rice J (eds) Organ and species specificity in chemical carcinogenesis. Plenum, New York, pp 129–156
Adamson RH, Krolikowski FJ, Correa P, Sieber SM, Dalgard DW (1977) Carcinogenicity of 1-methyl-1-nitrosourea in nonhuman primates. JNCI 59:415–422
Amo H, Kubota H, Lu J, Matsuyama M (1990) Adenomatous hyperplasia and adenomas in the lung induced by chronic feeding of butylated hydroxyanisole of Japanese house musk shrew (Suncus murinus). Carcinogenesis 11:151–154
Druckrey H (1975) Chemical carcinogenesis onN-nitroso derivatives. Gann Monogr 17:107–132
Herrold KM (1966) Epidermoid carcinomas of esophagus and forestomach induced in syrian hamsters byN-nitro-N-methylurethan. JNCI 37:389–394
Iizuka T, Ichimura S, Kawachi T (1980) Esophageal carcinoma in rats induced byN-amyl-N-methylnitrosamine. Gann 71:94–99
Kogure K, Sasadaira H, Kawachi T, Shimosato Y, Tokunaga A, Fujimura S, Sugimura T (1974) Further studies on induction of stomach cancer in hamsters byN-methyl-N′-nitro-N-nitrosoguanidine. Br J Cancer 29:132–142
Levison DA, Hopwood D, Morgan RGH, Coghill G, Milne GA, Wormsley KG (1979) Oesophageal neoplasia in male Wistar rats due to parenteral di(2-hydroxypropyl)-nitrosamine (DHPN): a combined histopathological, histochemical and electron microscopic study. J Pathol 129:31–36
Matsuzaki T, Tanaka T, Saito R, Yamanaka M, Saito M, Nomura T (1992) Establishment of an outbred strain (Jic: SUN) in the house musk shrew,Suncus murinus. Exp Anim 41:167–172
Moses FM (1991) Squamous cell carcinoma of the esophagus. Natural history, incidence, etiology, and complications. Gastroenterol Clin North Am 20:703–716
Muñoz N, Crespi M (1986) Precancerous lesions of the oesophagus and their risk factors. Gann Monogr 31:27–33
Napalkov NP, Pozharisski KM (1969) Morphogenesis of experimental tumors of the esophagus. JNCI 42:922–940
Odashima S (1979) Tumours of the oral cavity, pharynx, oesophagus and stomach. In: Turusov VS (ed) Pathology of tumours in laboratory animals, vol II. Tumours of the mouse. IARC Sci Publ 147–167
Ohgaki H, Hasegawa H, Kusama K, Morino K, Matsukura N, Sato S, Maruyama K, Sugimura T (1986) Induction of gastric carcinomas in nonhuman primates byN-ethyl-N′-nitro-N-nitrosoguanidine. JNCI 77:179–186
Ohgaki H, Kleihues P, Sugimura T (1991) Experimental gastric cancer. Ital J Gastroenterol 23:371–377
Pozharisski KM (1973) Tumours of the oesophagus. In: Turusov VS (ed) Pathology of tumours in laboratory animals, vol I. Part 1. Tumours of the Rat, IARC Scientific Publ 87–100
Romer AS, Parson TS (1978) The vertebrate body, 5th edn. Saunders, Philadelphia, pp 75–91
Sander J, Schweinsberg F (1973) Tumorinduktion bei Mäusen durchN-methylbenzyl-nitrosamin in niedriger Dosierung. Z Krebsforsch 79:157–161
Sasajima K, Kawachi T, Sano T, Sugimura T, Shimosato Y, Shirota A (1977) Esophageal and gastric cancers with metastases induced in dogs byN-ethyl-N′-nitro-N-nitrosoguanidine. JNCI 58:1789–1794
Sekizuka H, Doi H, Sunagawa M, Nagai S, Kojima S, Hiraide H, Hoshi K, Murakami T (1975) Induction of esophageal cancer associated with gastric cancer in a dog byN-ethyl-N′-nitro-N-nitrosoguanidine. Gann 66:683–688
Sharashidze LK, Beniashvili DSh, Sherenesheva NI, Turkia NG (1989) Induction of gastric cancer in monkeys byN-methyl-N′-nitro-N-nitrosoguanidine (MNNG). Neoplasma 36:129–133
Stinson SF (1979) Esophageal carcinoma in the rat induced with methylalkyl-nitrosamines. Am J Pathol 96:871–874
Sugimura T, Fujimura S (1967) Tumor productions in glandular stomach of rats byN-methyl-N′-nitro-N-nitrosoguanidine. Nature 216:943–944
Takubo K, Shisa H, Futatsuki K, Sasajima K (1981) Early changes of dog esophageal mucosa induced byN-ethyl-N′-nitro-N-nitrosoguanidine. Gann 72:880–885
Tsubura A, Senzaki H, Shikata N, Oishi Y, Morii S (1991) Induction of tumors in the Japanese house musk shrew,Suncus murinus (Insectivora), by 7,12-dimethylbenz[α]anthracene. Carcinogenesis 12:1433–1436
Tsubura A, Takahashi H, Morii S (1992) DMBA-induced intestinal tumors in the Japanese house musk shrew,Suncus murinus (Insectivora). In vivo 6:255–260
Tsubura A, Oyaizu N, Fujita Y, Asada M, Hilgers J, Morii S (1993) Keratin phenotypes in basal cell carcinomas of the skin. Int J Oncol 2:753–758
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tsubura, A., Senzaki, H., Takahashi, H. et al. Esophageal carcinoma in house musk shrews,Suncus murinus (insectivora), induced by N-methyl-N′-nitro-N-nitrosoguanidine. J Cancer Res Clin Oncol 119, 717–720 (1993). https://doi.org/10.1007/BF01195342
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01195342