Abstract
The serum antibody activity toPsoroptes ovis antigens, as determined by enzyme-linked immunosorbent assay (ELISA), was monitored during the development and decline phase of scabies in cattle herds under feedlot conditions in an endemic (Bushland) and a nonendemic (Kerrville) scabies area of Texas. The infestations were initiated by exposing six calves in each of the herds toP. ovis and allowing the infestations to spread to other members of the herd. The cattle in the endemic area developed more lesions and a higher incidence of the disease than did the cattle in the nonendemic area; also, there were more cattle with specific serum antibody activity in the Bushland herd than in the Kerrville herd at the peak of the infestations (late January-early February). As infestations declined because of summer latency, the number of calves with specific serum antibody activity declined in both herds. Specific serum antibody activity disappeared in the Kerrville cattle whenP. ovis was no longer detected. However, specific serum antibody activity was still detected in some of the Bushland cattle in late summer (August) and fall (October) examinations, when low numbers ofP. ovis were detected. These results indicate that the ELISA would be very useful in detection of specific serum antibody activity in lightly infested calves, especially during the developmental and decline phases of the disease.
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Allen, J.R., Khalil, H.M. and Graham, J.E., 1979a. The location of tick salivary antigens, complement and immunoglobulin in the skin of guinea-pigs infested withDermacentor andersoni larvae. Immunology, 38: 467–472.
Allen, J.R., Khalil, H.M. and Wikel, S.K., 1979b. Langerhans cells trap tick salivary gland antigens in tick-resistant guinea pigs. J. Immunol., 122: 563–565.
Bradshaw, L.J., 1966. Introduction to Molecular Biological Techniques. Prentice-Hall, Englewood Cliffs, NJ, pp. 118–120, 165.
Cole, N.A., Guillot, F.S. and Purdy, C.W., 1984. Influence ofPsoroptes ovis (Hering) (Acari: Psoroptidae) on the performance of beef steers. J. Econ. Entomol., 77: 390–393.
Farrell, C.J., Shen, D.T., Wescott, R.B. and Lang, B.Z., 1981. An enzyme-linked immunosorbent assay for diagnosis ofFasciola hepatica infection in cattle. Am. J. Vet. Res., 42: 237–240.
Fisher, W.F., 1983. Development of serum antibody activity as determined by enzyme-linked immunosorbent assay toPsoroptes ovis (Acarina: Psoroptidae) antigens in cattle infested withP. ovis. Vet. Parasitol., 13: 363–373.
Fisher, W.F. and Crookshank, H.R., 1982. Effects ofPsoroptes ovis (Acarina: Psoroptidae) on certain biochemical constituents of cattle serum. Vet. Parasitol., 11: 241–251.
Fisher, W.F. and Wilson, G.I., 1977. Precipitating antibodies in cattle infested byPsoroptes ovis (Acarina: Psoroptidae). J. Med. Entomol., 14: 146–151.
Fisher, W.F. and Wright, F.C., 1981. Effects of the sheep scab mite on cumulative weight gains in cattle. J. Econ. Entomol., 74: 234–237.
Guillot, F.S., 1981. Population increase ofPsoroptes ovis (Acari: Psoroptidae) on stanchioned cattle during summer. J. Med. Entomol., 18: 44–47.
Guillot, F.S. and Cole, N.A., 1984. Development and transmission of psoroptic mange of cattle in feed lots in endemic and non-endemic regions. Vet. Parasitol., 16: 127–135.
Heck, F.C., Deyoe, B.L. and Williams, J.D., 1982. Antibodies toBrucella abortus in sera from strain 19 vaccinated and non-vaccinated cows as determined by enzyme linked immunosorbent assay and conventional serologic methods. Vet. Immunol. Immunopathol., 3: 629–634.
Hourrigan, J.L., 1979. Spread and detection of psoroptic scabies of cattle in the United States. J. Am. Vet. Med. Assoc., 175: 1278–1280.
Kelley, K.W., 1980. Stress and immune function: a bibliographic review. Ann. Rech. Vet., 11: 445–478.
Kemper, H.E. and Peterson, H.O., 1953. Cattle scab and methods of control and eradication. USDA Farmers' Bull. 1017, 26 pp.
Meleney, W.P. and Christy, J.E., 1978. Factors complicating the control of psoroptic scabies of cattle. J. Am. Vet. Med. Assoc., 173: 1473–1478.
Meleney, W.P. and Fisher, W.F., 1979. The effect of exposure to mites,Psoroptes ovis (Acarina: Psoroptidae), on calves of varying susceptibility to common scabies. Proc. Annu. Meet. U.S. Anim. Health Assoc., 83: 340–346.
Pruett, J.H., Guillot, F.S. and Fisher, W.F., 1986. Humoral and cellular immunoresponsiveness of stanchioned cattle infested withPsoroptes ovis. Vet. Parasitol. (in press).
Roberts, I.H. and Meleney, W.P., 1971. Variations among strains ofPsoroptes ovis (Acarina: Psoroptidae) on sheep and cattle. Ann. Entomol. Soc. Am., 64: 109–116.
Schubert, G., 1981. Scabies eradication. Proc. Ann. Meet. Livestock Conserv. Inst. (May 26–28), pp. 120–125.
Seawright, G.L., Sanders, W.M. and Bryson, M., 1981. Automation of the enzyme immunoassay for the serodiagnosis of infectious diseases in livestock. In: J.E. Butler (Editor), The Ruminant Immune System. Plenum, New York, NY, pp. 145–168.
Tobin, W.C., 1962. Cattle scabies can be costly. J. Am. Vet. Med. Assoc., 141: 845–847.
Tsang, V.C.W., Wilson, B.C. and Maddison, S.E., 1980. Kinetic studies of a quantitative single-tube enzyme-linked immunosorbent assay. Clin. Chem., 26: 1255–1260.
Voller, A., Bidwell, D. and Bartlett, A., 1980. Enzyme-linked immunosorbent assay. In: N.R. Rose and H. Friedman (Editors), Manual of Clinical Immunology, 2nd Edition. American Society for Microbiology, Washington, DC, pp. 359–371.
Willadsen, P. and McKenna, R.V., 1983. Bindin of antigens to tissues: the example ofBoophilus microplus and bovine skin. Int. J. Parasitol., 13: 593–598.
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Fisher, W.F., Guillot, F.S. & Cole, N.A. Development and decline of serum antibody activity toPsoroptes ovis antigens in infested cattle in an endemic and nonendemic scabies area of texas. Exp Appl Acarol 2, 239–248 (1986). https://doi.org/10.1007/BF01193956
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DOI: https://doi.org/10.1007/BF01193956