Summary
Tumour necrosis factor α (TNFα) exerts cytotoxic and antiproliferative effects on neoplastic cells. It has been used as a therapeutic agent for solid tumours and haematological malignancies. We report on the ex vivo determination of the effect of recombinant human rhuTNFα on bone marrow aspirates by a bromodeoxyuridine/propidium iodide method. Cell samples were drawn after 0.5, 2, 4, 6, 8, 10, 22, and 25 h from shortterm suspension bone marrow cultures from patients with acute myelogenous leukemia (AML). Flow-cytometric cell-cycle analysis was performed after double DNA staining with propidium iodide and anti-BrdU antibodies. By this method the effect of rhuTNFα on cell proliferation can be evaluated after only 35 h. In about two-thirds of the bone marrow aspirates of AML an inhibiting effect on rhuTNFα can be demonstrated, developing to its full extent after 10 h.
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Abbreviations
- TNFα:
-
tumour necrosis factor α
- AML:
-
acute myelogenous leukemia
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Kleine, H.D., Wagner, U., Poliwoda, H. et al. Effects of tumour necrosis factor α on bone marrow aspirates of patients with acute myelogenous leukemia determined by flow-cytometric cell-cycle analysis. J Cancer Res Clin Oncol 118, 56–60 (1992). https://doi.org/10.1007/BF01192312
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DOI: https://doi.org/10.1007/BF01192312