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On the course of thermal denaturation of deoxyribonucleic acids after γ-irradiation

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Summary

The changes which are caused by action ofγ-irradiation onDNAs of various origin were followed by spectrophotometric method at differing thermal denaturation curves. It was found that all measured bacterialDNAs as well asDNA isolated from calf thymus, irradiated by exposures higher that 5×104 R, produced significantly decreasedT m values with concomittantly decreased hyperchromic effect and changed transition intervals obtained in 10−2 M sodium phosphate, 10−3 M EDTA medium at pH 7. It was also observed that higherγ-irradiation exposures caused the loss of renaturation ability ofEscherichia coli DNA.

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Abbreviations

DNA :

deoxyribonucleic acid

G :

guanine

C :

cytosine

E 260 :

extinction (absorbancy) measured at 260mμ

T m :

the temperature corresponding to the midpoint of the absorbance rise

Δ2/3:

the transition interval of the denaturation curve corresponding to the temperature interval between 17–83% of the total absorbancy increment of the denaturation curve

SSC :

standard saline citrate buffer (0.15 M NaCl, 0.015 M sodium citrate, pH 7)

PE :

10−2 M sodium phosphate buffer [molarity related to (PO4)]

PE :

10−3 M EDTA, pH 7. 1.000 ml prepared as follows: 0.608 g NaH2PO4.2 H2O, 0.218 g Na2HPO4.12 H2O, 0.372 g disodium salt of EDTA, 1.0 ml 1 M NaOH. Concentration of Na ions — 0.02 M.

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Authors and Affiliations

  1. Institute of Biophysics, Czechoslovak Academy of Sciences, Brno

    Jiří Boháček & Gustav Blažíček

Authors
  1. Jiří Boháček
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  2. Gustav Blažíček
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Boháček, J., Blažíček, G. On the course of thermal denaturation of deoxyribonucleic acids after γ-irradiation. Biophysik 2, 233–238 (1965). https://doi.org/10.1007/BF01191673

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  • DOI: https://doi.org/10.1007/BF01191673

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