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Helical regions in transfer ribonucleic acid

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Summary

By following the thermal denaturation of transfer RNA (t-RNA) over a wide wavelength range it can be demonstrated that t-RNA dissociates in at least two steps. The lower melting step seems to melt cooperatively, while this can not be stated with confidence about the higher melting region. It is shown that the use of difference spectra of two-stranded transitions gives similar, but different results than the use of difference spectra for three stranded-transitions. It is thus shown that difference spectra can not be used (at least at present) to distinguish strandedness in RNA. Possible applications of the method of difference spectra are discussed.

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Authors and Affiliations

  1. Institut de Biologie Physico-Chimique, 13, rue Pierre Curie, Paris V, France

    Wilhelm Guschlbauer

  2. Département de Biologie, Centre d'Etudes, Nucléaires de Saclay, 91 Gif sur Yvette, B.P. No. 2, France

    Wilhelm Guschlbauer

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  1. Wilhelm Guschlbauer
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Guschlbauer, W. Helical regions in transfer ribonucleic acid. Biophysik 3, 156–164 (1966). https://doi.org/10.1007/BF01191609

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  • DOI: https://doi.org/10.1007/BF01191609

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