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Biophysik

, Volume 4, Issue 3, pp 233–242 | Cite as

Zur Isolierung einer strahlensensiblen Saccharomyces-Mutante Empfindlichkeit des Koloniebildungsvermögens, der RNS- und Proteinsythese

  • W. Laskowski
  • E. -R. Lochmann
  • S. Jannsen
  • E. Fink
Article

Summary

A radiation-sensitive strain has been isolated fromSaccharomyces. By tetrad analysis it has been shown that the radiation-sensitivity is due to a gene mutation. The mutated gene, r 1 s , makes haploid cells 3 to 6 times more sensitive to UV and approximately twice as sensitive to X-rays. Homozygous diploid cells (r 1 s r 1 s ) are approximately 2,5 times more sensitive to UV and X-rays than wild-type cells (++). In both haploid and homozygous diploid cells photo-reactivation and dark-recovery on agar without nutrients are not blocked by gene r 1 s . In comparison to wild-type strains, the rate of RNA-synthesis in the radiation-sensitive strains is reduced after UV- and after X-irradiation whereas the rate of protein-synthesis is reduced only after UV-irradiation.

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Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • W. Laskowski
    • 1
  • E. -R. Lochmann
    • 1
  • S. Jannsen
    • 1
  • E. Fink
    • 1
  1. 1.Institut für Biophysik der Freien Universität BerlinDeutschland

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