Abstract
SCEs were studied in the chromosomes of Allium cepa L. stained by the FPG technique at the second and third divisions after BrdU-substitution during only the first replication round or the three consecutive cycles, respectively. Cells were cultured in the dark and exposed to visible light at different moments throughout the three cycles. The results obtained show that visible light-illumination, which has no apparent effect upon native DNA, is able to increase the frequency of SCEs in BrdU-substituted chromosomes. The comparison of expected and observed figures clearly reveals that BrdU-substituted DNA is the target of visible light. Finally, the formation of visible light-induced SCEs in BrdU-substituted chromosomes appears to be an S-dependent process, even though a post-replicational mechanism closely associated with semi-conservative S phase replication might be responsible.
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Abbreviations
- SCE:
-
sister chromatid exchange
- BrdU:
-
5-bromodeoxyuridine
- Thd:
-
thymidine
- FdU:
-
5-fluorodeoxyuridine
- Urd:
-
uridine
- FPG:
-
fluorescent plus Giemsa
- UV:
-
ultraviolet
- CHO:
-
Chinese hamster ovary
References
Ben-Hur, E., Elkind, M.M.: Damage and repair of DNA in 5-bromodeoxyuridine-labelled Chinese hamster cells exposed to fluorescent light. Biophys. J.12, 636–647 (1972)
Bostock, C.J., Sumner, A.T.: The eukaryotic chromosome. Amsterdam: North Holland 1978
Brookes, P., Lawley, P.: The reaction of mono- and di-functional alkylating agents with nucleic acids. Biochem J.80, 496–503 (1961)
Cerutti, P.A.: Repairable DNA damage: Overview. In: Basic life sciences (A. Hollaender, ed.), Vol. 5A, pp. 3–12. New York: Plenum Press 1975
Evans, H.J.: What are sister chromatid exchanges? In: Chromosomes today6, 315–326 (1977)
Gonzalez-Fernandez, A., Lopez-Saez, J.F., Giménez-Martín, G.: Duration of the division cycle in binucleate and mononucleate cells. Exp. Cell Res.43, 255–267 (1966)
Goth-Goldstein, R.: Repair of DNA damaged by alkylating carcinogens is defective in xeroderma pigmentosum-derived fibroblasts. Nature (Lond.)267, 81–82 (1977)
Hanawalt, P., Grivell, A., Nakayama, H.: DNA turnover and strand breaks in Escherichia coli. In: Basic life sciences (A, Hollaender, ed.), Vol. 5A, pp. 47–50. New York: Plenum Press 1975
Ikushima, T.: Role of sister chromatid exchanges in chromatid aberration formation. Nature (Lond.)268, 235–236 (1977)
Ikushima, T., Wolff, S.: Sister chromatid exchanges induced by light flashes to 5-bromodeoxyuridine- and 5-iododeoxyuridine-substituted chromosomes of Chinese hamster. Exp. Cell Res.87, 15–19 (1974)
Kato, H.: Possible role of DNA synthesis in formation of sister chromatid exchanges. Nature (Lond.)252, 739–741 (1974)
Kato, H.: Spontaneous and induced sister chromatid exchanges as revealed by the BUdR-labelling method. Int. Rev. Cytol.49, 55–97 (1977)
Kihlman, B.A., Sturelid, S.: Effects of caffeine on the frequencies of chromosomal aberrations and sister chromatid exchanges induced by chemical mutagens in root tips of Vicia faba. Hereditas (Lund)88, 35–41 (1978)
Kihlman, B.A., Natarajan, A.T., Andersson, H.C.: Use of 5-bromodeoxyuridine-labelling technique for exploring mechanisms involved in the formation of chromosomal aberrations. I. G2 experiments with root tips of Vicia faba. Mutation Res.52, 181–198 (1978)
Kuebbing, D., Werner, R.: A model for compartmentation of de novo and salvage thymidine nucleotide pools in mammalian cells. Proc. nat. Acad. Sci. (Wash.)72, 3333–3336 (1975)
Lehmann, A.R.: Post-replication repair of DNA in UV-irradiated mammalian cells. In: Basic life sciences (A. Hollaender, ed.), Vol. 5B, pp. 617–623. New York: Plenum Press 1975
Lehmann, A.R., Bridges, B.A.: DNA repair. Essays Biochem.13, 71–119 (1977)
Lindahl, T., Ljungquist, S.: Apurinic and apyrimidinic sites in DNA. In: Basic life sciences (A. Hollaender, ed.), Vol. 5A, pp. 31–38. New York: Plenum Press 1975
Mazrimas, J.A., Stetka, D.G.: Direct evidence for the role of incorporated BUdR in the induction of sister chromatid exchanges. Exp. Cell Res.117, 23–30 (1978)
Miller, R.C., Aronson, M.M., Nichols, W.W.: Effect of treatment on differential staining of BrdUlabelled metaphase chromosomes: three way differentiation of M3 chromosomes. Chromosoma (Berl.)55, 1–11 (1976)
Palitti, F., Becchetti, A.: Effect of caffeine on sister chromatid exchanges and chromosomal aberrations induced by mutagens in Chinese hamster cells. Mutation Res.45, 157–159 (1977)
Rommelaere, J., Suskind, M., Errea, M.: Chromosomes and chromatid exchanges in Chinese hamster cells. Chromosoma (Berl.)41, 243–257 (1973)
Sasaki, M.: Sister chromatid exchange and chromatid interchange as possible manifestation of different DNA repair processes. Nature (Lond.)269, 623–625 (1977)
Schvartzman, J.B., Cortes, F.: Sister chromatid exchanges in Allium cepa L. Chromosoma (Berl.)62, 119–131 (1977)
Schvartzman, J.B., Cortes, F., Lopez-Saez, J.F.: Sister subchromatid exchanged segments and chromosome structure. Exp. Cell Res.114, 443–446 (1978)
Schvartzman, J.B., Cortes, F., Gonzalez-Fernandez, A., Gutierrez, C., Lopez-Saez, J.F.: On the nature of sister chromatid exchanges in 5-bromodeoxyuridine-substituted chromosomes. Genetics (in press, 1979)
Strauss, B., Coyle, M., Robbins, M.: Alkylation damage and its repair. Cold Spr. Harb. Symp. quant. Biol.33, 277–287 (1968)
Ward, J.F.: Radiation-induced strand breakage in DNA. In: Basic life sciences (A. Hollaender, ed.), Vol. 5B, pp. 471–472. New York: Plenum Press 1975
Wolff, S.: Sister chromatid exchange. Ann. Rev. Genet.11, 183–201 (1977)
Wolff, S., Perry, P.: Differential Giemsa staining of sister chromatids and the study of sister chromatid exchanges without autoradiography. Chromosoma (Berl.)48, 341–353 (1974)
Wolff, S., Bodycote, J., Painter, R.B.: Sister chromatid exchanges induced in Chinese hamster cells by UV-irradiation at different stages of the cell cycle: the necessity for cells to pass through S. Mutation Res.25, 73–81 (1974)
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Schvartzman, J.B., Postigo, R. & Gutiérrez, C. Analysis of visible light-induced sister chromatid exchanges in 5-bromodeoxyuridine-substituted chromosomes. Chromosoma 74, 317–328 (1979). https://doi.org/10.1007/BF01190746
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DOI: https://doi.org/10.1007/BF01190746