Summary
The dark resistance and capacitance of oxidised cholesterol Black Lipid Membrane was measured in presense of acidic and basic dyes. The resistance of the membrane decreases almost 10-fold while the capacitance increases with increasing concentration of dyes. The results have been interpreted in terms of solubilization of the dyes by the Black Lipid Membrane. Exposure of the BLMs containing dyes to visible light filtered through 5 % CuSO4 solution, produced trans membrane emf. These photo-emfs are attributed to the production of charge carriers and their subsequent separation. Similarities between the dye modified BLMs and dye coated electrodes have been noted.
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References
Bangham, A. D.: Membrane models with phospholipids. Progr. Biophys. Moleo. Biol. (Butler, J. A. V., Noble, D., Eds)18, 29–95 (1968).
Bard, J. A., Ed.: Electroanalyt. Chem.I, 214 (1966).
Finkelstein, A., Cass, A.: Permeability and electrical properties of thin Iipid membranes. J. gen. Physiol.52, 145–173 (1968).
Goldup, A., Ohki, S., Danielli, J. F.: Black lipid films. Recent Progr. Surface Science (Danielli, J. F., Biddiford, A. C, Rosenberg, M. D., Eds) 3, 193–260 (1970).
Kallmann, H., Pope, M.: Positive hole injection into organic crystals. J. Chem. Phys.32, 300–301 (1960).
Kruyt, H. R., (Ed.): Colloid ScienceI, 135 (1952).
Lauger, P., Lesslaur, W., Marti, E., Richter, J.: Electrical properties of bimolecular phospholipid membranes. Biochim. biophys. Acta (Amst.)135, 20–32 (1967).
Mauzerall, D., Finkelstein, A.: Light induced changes in the conductivity of thin Iipid membranes in the presence of iodine and iodide ion. Nature (Lond.)224, 690–692 (1969).
Müller, P., Rudin, D. O., Tien, H. T., Wescott, W. C.: Reconstitution of excitable cell membrane structurein vitro. Symposium on the Plasma Membrane (Fishman, A. P., Ed.). Circulation26, 1167–1171 (1962).
Szent-Györgyi, A. V.: Über den Mechanismus der Succin- und Paraphenylendiaminoxyda-tion. Ein Beitrag zur Theorie der Zellatmung. Biochem. Z.150, 195–210 (1924).
Tien, H. T.: Photoelectric effects in thin and bilayer lipid membranes in aqueous media. J. Phys. Chem.72, 4512–4518 (1968).
—, Diana, A. L.: Bimolecular lipid membranes: a review and a summary of some recent studies. Chem. Phys. Lipid2, 55–101 (1968).
— —: Black lipid membranes in aqueous media: the effect of salts on electrical properties. J. Colloid Inter. Sci.24, 287–296 (1967).
—, Kobamoto, N.: Carotenoid bilayer lipid membrane model for visual receptor. Nature (Lond.)224, 1107–1111 (1969).
—, Ting, H. P.: Permeation of water through bilayer lipid membranes. J. Colloid Inter. Sci.27, 702–713 (1968).
—, Verma, S. P.: Electronic processes in bilayer lipid membranes. Nature (Lond.)227, 1232 (1970).
Verma, S. P.: Photo-induced redox reactions at the surface of bilayer lipid membranes. (In press. Ind. J. Chem.).
- Black lipid membrane as an electrode. Unpublished.
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These investigations were supported by grants from the U.S. National Institutes of Health and the Office of Saline Water.
The author is thankful to Professor Dr. H. T. Tien for his helpful suggestions during this work.
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Veema, S.P. Photo-sensitivity of oxidised cholesterol black lipid membrane incorporated with acid and basic dyes. Biophysik 7, 228–235 (1971). https://doi.org/10.1007/BF01190730
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DOI: https://doi.org/10.1007/BF01190730