Abstract
Conditions were chosen for optimal demonstration of ATPases in brain slices by a modified method of Wachstein and Meisel, and the reaction was shown to obey the Bouguer-Beer laws, confirming that ATPase activity can be determined quantitatively in single cells by cytophotometry. In rats in a state of relative physiological rest specific activity of both Na+, K+-ATPase and Mg++-ATPase in gliocytes of the hippocampus and dorsal raphe was found to be considerably higher than in neurons. Deprivation of the paradoxical phase of sleep of the rats for 24 h led to a significant increase in Na+, K+-ATPase activity in hippocampal neurons and to a decrease in its activity in gliocytes of the hippocampus and dorsal nucleus raphe. It is suggested that these changes in Na+, K+-ATPase activity may be due to some extent to a change in excitability of neurons and depolarization of the glia when sleep is disturbed.
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Translated from Fiziologicheskii Zhurnal SSR imeni I. M. Sechenova, Vol. 74, No. 4, pp. 490–496, April, 1988.
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Klenikova, V.A., Taranova, N.P. Cytophotometric study of ATPase activity in brain neurons and gliocytes of paradoxical sleep-deprived rats. Neurosci Behav Physiol 19, 163–169 (1989). https://doi.org/10.1007/BF01190463
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DOI: https://doi.org/10.1007/BF01190463