Skip to main content
SpringerLink
Log in

Instantaneous reactions of guinea-pig vas deferens preparation to X-irradiation

General radiobiological studies

  • Published:
Biophysik Aims and scope Submit manuscript

Summary

In the mechanical-inactive guinea-pig vas deferens X-rays (25 kV) at threshold doses of about 100 kR initiated phasic activity and an immediate increase in tone. After addition of acetylcholine, noradrenaline or adrenaline to the rinsing solution a slight contractile activity of vas deferens appeared and the preparation reacted to X-irradiation at a threshold dose of 3 to 5 kR (dose-rate 20 kR/min) with increased phasic contractions and with a dose and dose-rate dependent tonic contraction. After repeated irradiation a sensitization was observed. X-rays produced tonic contractions of the vas deferens preparation up to a total dose of about 200 kR (fractionated irradiation). An irreversible contraction of the vas appeared after continuous exposure to X-ray doses larger than 500 kR (dose-rate 20 kR/min).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bacq, Z. M., Lecomte, J., Herve, A.: Action des radiations ionisantes sur le muscle strié de grenouille. Arch. int. Physiol.57, 142 (1949).

    Google Scholar 

  2. Bergeder, H.-D.: Untersuchungen zum Einfluß ionisierender Strahlen auf Zellmembranen. II. Änderungen von Membranpotential und Kaliumgehalt des Muskels unter Einwirkung von Röntgenstrahlen. Strahlentherapie115, 90 (1961).

    Google Scholar 

  3. Bhargava, K. P., Kar, K., Parmar, S. S.: Independent cholinergic and adrenergic mechanisms in the guinea-pig isolated nerve vas deferens preparation. Brit. J. Pharmacol.24, 641 (1965).

    Google Scholar 

  4. Birmingham, A. T.: Sympathetic denervation of the smooth muscle of the vas deferens. J. Physiol. (Lond.)206, 645 (1970).

    Google Scholar 

  5. Burn, J. H., Kordik, P., Mole, R. H.: The effect of X-irradiation on the response of intestine to acetylcholine and its content of “pseudo”-cholinesterase. Brit. J. Pharmacol.7, 58 (1952).

    Google Scholar 

  6. —, Weetman, D. F.: The effect of eserine on the response of the vas deferens to hypogastric nerve stimulation. Brit. J. Pharmacol.20, 74 (1963).

    PubMed  Google Scholar 

  7. Conard, R. A.: Some effects of ionizing radiation on the physiology of the gastrointestinal tract: a review. Radiat. Res.5, 167 (1956).

    PubMed  Google Scholar 

  8. Falck, B., Owman, Ch., Sjöstrand, N. O.: Peripherally located adrenergic neurons innervating the vas deferens and the seminal vesicle of the guinea-pig. Experientia (Basel)21, 98 (1965).

    Google Scholar 

  9. Ferry, C. B.: The innervation of the vas deferens of the guinea-pig. J. Physiol. (Lond.)192, 463 (1967).

    Google Scholar 

  10. Grutschkowski, E.: Die Wirkung von Röntgenstrahlen auf den Tonus der isolierten Froschlunge. Diss., Univ. München 1967.

  11. Hug, O., Schliep, H.-J.: Immediate reactions of nerves and muscles to ionizing radiation. Symposium on the Initial Effects of Ionizing Radiations on Cells, Moscow, p. 287. New York: Academic Press L. 1960.

    Google Scholar 

  12. Huković, S.: Responses of the isolated sympathetic nerve-ductus deferens preparation of the guinea-pig. Brit. J. Pharmacol.16, 188 (1961).

    PubMed  Google Scholar 

  13. Large, B. J.: Sympatheticβ-receptors and the guinea-pig vas deferens. Brit. J. Pharmacol.24, 194 (1965).

    Google Scholar 

  14. McEwen, I. M.: The effect on the isolated rabbit heart of vagal stimulation and its modification by cocaine, hexamethonium and ouabain. J. Physiol. (Lond.)131, 678 (1956).

    Google Scholar 

  15. Merrillees, N. C. R., Burnstock, G., Holman, M. E.: Correlation of fine structure and physiology of the innervation of smooth muscle in the guinea-pig vas deferens. J. Cell Biol.19, 529 (1963).

    PubMed  Google Scholar 

  16. Michailov, M. Ch.: Instantaneous reactions of guinea-pig taenia coli, vas deferens and vesica urinaria to ionizing and optical radiation. IV. Intern. Congress of Radiation Research, Evian. Book of Abstracts, p. 145, 1970.

  17. Michailov, M. Ch.: Immediate reactions of isolated guinea-pig and rat urinary bladder to X-irradiation. Strahlentherapie139, 330 (1970).

    PubMed  Google Scholar 

  18. - Sofortreaktion der glatten Muskulatur von Säugetieren auf Röntgenbestrahlung. Deutscher Röntgenkongress München, 21.–24. Mai 1970 (Autoreferate).

  19. Ohlin, P., Strömblad, B. C. R.: Observations on the isolated vas deferens. Brit. J. Pharmacol.20, 299 (1963).

    PubMed  Google Scholar 

  20. Sjöstrand, N. O.: Inhibition by ganglionic blocking agents of the motor response of the isolated guinea-pig vas deferens to hypogastric nerve stimulation. Acta physiol. scand.54, 306 (1962).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Biologie, Abteilung für Strahlenbiologie und Biophysik, Gesellschaft für Strahlen- und Umweltforschung mbH München, Neuherberg

    M. Ch. Michailov

Authors
  1. M. Ch. Michailov
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Michailov, M.C. Instantaneous reactions of guinea-pig vas deferens preparation to X-irradiation. Biophysik 7, 276–282 (1971). https://doi.org/10.1007/BF01190239

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01190239

Keywords

Search

Navigation