Skip to main content
SpringerLink
Log in

Fluorometric method for determination of vitamin B6 in soya bean

Die fluorometrische Methode zur Vitamin B6-Bestimmung in Soja

  • Original Paper
  • Published:
Zeitschrift für Lebensmittel-Untersuchung und Forschung Aims and scope Submit manuscript

Zusammenfassung

Die einfache fluorometrische Methode zur Bestimmung von zugegebenem Pyridoxin in angereicherten Lebensmitteln basiert auf der Oxidation des Pyridoxins mit Kaliumpermanganat zur 4-Pyridoxinsäure und wurde für die Bestimmung des gesamten Vitamin B6 in Soja modifiziert. Im Vergleich mit anderen fluorometrischen Methoden ist die vorgeschlagene, einfach, schnell und leicht durchzuführen. Diese Methode braucht nur wenig Zeit für die Oxidation und verwendet ungiftige Chemikalien (KMnO4 statt KCN). Die gute Wiederfindung, die geringe relative Standardabweichung und der geringe Gesamtfehler reiht das vorgeschlagene Verfahren in die Gruppe der ausgezeichneten Methoden ein.

Summary

The simple fluorometric method for determination of added pyridoxine-HCl in enriched food, based on oxidation of pyridoxine to 4-pyridoxic acid by means of KMnO4, has been modified for determination of total B6 in soya bean. In comparison to the other fluorometric procedures, the proposed method is the simplest one, rapid and easy to perform, demanding the least time for oxidation and not using hazardous chemicals (KMnO4 instead of KCN). Good recovery, low relative standard deviation and low total error allow the proposed procedure to be included into the group of excellent methods.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Bonavita V, Scardi V (1959) Anal Chim Acta 20:47–50

    Google Scholar 

  2. Bonavita V (1960) Arch Biochem Biophys 88:366–372

    PubMed  Google Scholar 

  3. Toepfer EW, MacArthur Polansky M, Hewston EM (1961) Anal Biochem 2:463–4690

    PubMed  Google Scholar 

  4. Toepfer EW, MacArthur Polansky M (1964) Vitam Horm (NY) 22:825–832

    Google Scholar 

  5. Fiedlerova V, Davidek J (1974) Z Lebensm Unters Forsch 155:277–281

    Google Scholar 

  6. Gregory JF, Kirk JR (1977) J Food Sci 42:1073–1076

    Google Scholar 

  7. Fiedlerova V, Davidek J (1978) Z Lebensm Unters Forsch 166:93–95

    PubMed  Google Scholar 

  8. Metzler DE, Olivard J, Snell EE (1954) J Am Chem Soc 76:644–648

    Google Scholar 

  9. MacArthur Polansky M, Camara RT, Toepfer EW (1964) J Assoc Off Agric Chem 47:827–830

    Google Scholar 

  10. Fujita A, Matsuura K, Fujino K (1955) J Vitaminol 1:267–274

    Google Scholar 

  11. Fujita A, Fujita D, Fujino K (1955) J Vitaminol 1:275–279

    Google Scholar 

  12. Fujita A, Fujita D, Fujino K (1955) J Vitaminol 1:279–289

    Google Scholar 

  13. Fujita A, Fujino K (1955) J Vitaminol 1:290–297

    Google Scholar 

  14. MacArthur MJ, Lehmann J (1959) J Assoc Off Agric Chem 42:619–622

    Google Scholar 

  15. Hennessy DJ, Steinberg AM, Wilson GS, Keaveney WP (1960) J Assoc Off Agric Chem 43:765–768

    Google Scholar 

  16. Strohecker R, Henning HM (1963) Vitamin Bestimmungen. Verlag Chemie, Weinheim, p 141

    Google Scholar 

  17. Horwitz W (1975) Official methods of analysis of the association of official analytical chemists, 12th edn. AOAC Washington, DC, pp 849–851

    Google Scholar 

  18. Hodson AZ (1956) Food Technol 10:221–224

    Google Scholar 

  19. Hausheer W, Moor H, Nobile S, Wagner H (1960) Vitaminbestimmungen in Lebensmitteln mit chemisch-physikalischen Methoden. Hoffman-La Roche, Basel, p 31

    Google Scholar 

  20. Driskell JA (1984) Vitamin B6. In: Machlin LJ (ed) Handbook of vitamins: nutritional, biochemical and clinical aspects, vol 13. Dekker, New York-Basel, p 379

    Google Scholar 

  21. Šebečić B, Vedrina-Dragojević I (1986) Nahrung 30:527–532

    PubMed  Google Scholar 

  22. Vedrina-Dragojević I, Šebečić B (1986) Z Lebensm Unters Forsch 182:127–130

    PubMed  Google Scholar 

  23. Vedrina-Dragojević I, Šebečić B, Balint L (1989) Nahrung 33:1017–1019

    PubMed  Google Scholar 

  24. Vedrina-Dragojević I, Momirović-Čuljat J, Balint L (1985) Z Acker- und Pflanzenbau 154:73–81

    Google Scholar 

  25. Vedrina-Dragojević I (1991) Z Acker Pflanzenbau (in press)

  26. Fragner J (1964) Vitamine: Chemie und Biochemie. Gustav Fischer, Jena, p 1329

    Google Scholar 

  27. Smith AK, Cirkle SJ (1972) Soybeans: chemistry and technology, vol 1. AVI, INC Westport, Connecticut, p 226

    Google Scholar 

  28. McFarren EF, Lishka RJ, Parker JH (1970) Anal Chem 42:358–365

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Food Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, Domagojeva 2, YU-41000, Zagreb, Croatia, Yugoslavia

    Blaženka Šebečić & Irena Vedrina-Dragojević

Authors
  1. Blaženka Šebečić
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Irena Vedrina-Dragojević
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Šebečić, B., Vedrina-Dragojević, I. Fluorometric method for determination of vitamin B6 in soya bean. Z Lebensm Unters Forch 194, 144–147 (1992). https://doi.org/10.1007/BF01190186

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01190186

Keywords

Search

Navigation