, Volume 7, Issue 2, pp 115–119 | Cite as

Electron spin resonance studies of ultraviolet irradiation effects on some proteins

  • I. M. Pepe


Electron spin resonance signals of dry protein samples are enhanced after ultraviolet irradiation at wavelength 3650 Å, which corresponds closely to the energy gap between valence and conduction bands.

Proteins denatured with short wave ultraviolet or 8 M urea fail to show the enhancement after irradiation at wavelength 3650 Å. This result supports the hypothesis that hydrogen bond structures are relevant in determining the semiconductor properties of proteins.


Urea Hydrogen Bond Electron Spin Resonance Conduction Band Protein Sample 
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  1. Allen, B. T., Ingram, D. J. E.: The investigation of unpaired electron concentrations produced in large molecules by ultraviolet irradiation. In: Free radicals in biological systems, p. 215. (Blois, M. S., Jr., Ed.) New York, London: Academic Press 1961.Google Scholar
  2. Cardew, M. H., Eley, D. D.: The semiconductivity of organic substances. Disc. Faraday Soc.27, 115–127 (1959).Google Scholar
  3. Dunlop, J. I., Nicholls, C. H.: Unpaired electrons in ultraviolet irradiated keratin. Nature (Lond.)205, 1310–1311 (1965).Google Scholar
  4. Evans, M. G., Gergely, J.: A discussion of the possibility of bands of energy levels in proteins. Biochim. biophys. Acta (Amst.)3, 188–197 (1949).Google Scholar
  5. Longuet-Higgins, H. C.: On the origin of the free radical property of melanins. Arch. Biochem.86, 231–232 (1960).PubMedGoogle Scholar
  6. Snart, R. S.: Effect of carcinogens and related compounds on the semiconductive properties of protein. Biopolymers6, 73–79 (1968).PubMedGoogle Scholar
  7. Szent-Györgyi, A.: The study of energy-levels in biochemistry. Nature (Lond.)148, 157–159 (1941).Google Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • I. M. Pepe
    • 1
    • 2
  1. 1.Sezione del Gruppo Nazionale di Cibernetica del C.N.R., Istituto di FisicaUniversità di GenovaItalia
  2. 2.International Laboratory of Genetics and BiophysicsNapoliItaly

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