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Biophysik

, Volume 7, Issue 2, pp 106–114 | Cite as

X-ray effects on ferredoxin fromClostridium pasteurianum

  • V. Krasnobajew
Article

Summary

X-ray irradiation of aqueous ferredoxin solutions isolated fromClostridium pasteurianum causes a rapid destruction of the ferredoxin molecule.

The destruction is manifested by the decrease of the absorption at 390 nm, the liberation of ferric iron and hydrogen sulfide, and concommittant loss of biological activity in the phosphoroclastic reaction ofC. pasteurianum. The biological activity decreases parallel with the iron liberation, and was found to be dose and pH dependent.

The yield of biological inactivation (G-value) and the yield of iron liberation showed the same value of 0.8. OH-radical scavengers like p-amino-benzoic acid and cysteine in low concentrations of 2×10−3 M, protect ferredoxin effectively against radiation, suggesting that OH-radicals are mainly responsible for the inactivation.

Keywords

Hydrogen Iron Sulfide Cysteine Biological Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • V. Krasnobajew
    • 1
  1. 1.Strahlenbiologisches Institut der Universität ZürichSchweiz

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