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Tumours induced by Moloney murine sarcoma virus are clonal in rats, not clonal in mice

  • Original Papers
  • Experimental Oncology
  • Published:
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Summary

Moloney murine sarcoma virus (M-MSV) induces rapidly growing tumours in adult mice of most conventional strains. Rats are less susceptible to M-MSV oncogenesis, but the few rhabdomyosarcomas that do develop after viral inoculation of newborn animals closely resemble conventional malignancies: they develop after a long latency, grow progressively, and metastasize to regional lymph nodes and lungs. Southern blot analysis with a v-mos-specific probe of M-MSV-induced tumours in both species demonstrated an oligo-, monoclonal pattern of exogenous v-mos integration only in the rat system, while mouse tumours were not clonal in origin. Furthermore, the same type of analysis of lymph node and lung metastases showed that cell clones already present in the primary rat lesion colonized secondary sites during tumour progression. Apparently, Moloney murine leukemia virus (M-MuLV) was not involved in rhabdomyosarcoma pathogenesis since M-MuLV-specific DNA sequences could not be demonstrated in three of the six rat tumours. Finally, in all mouse tumours, unintegrated linear M-MSV proviruses could be readily detected.

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Abbreviations

M-MSV:

Moloney murine sarcoma virus

M-MuLV:

Moloney murine leukemia virus

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Authors and Affiliations

  1. Institute of Oncology, Interuniversity Center for Cancer Research (C.I.R.C.), University of Padova, Via Gattamelata 64, 35128, Padova, Italy

    Emma D'Andrea, Lino Tessarollo, Chiara Menin & Luigi Chieco-Bianchi

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  1. Emma D'Andrea
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  2. Lino Tessarollo
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  3. Chiara Menin
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  4. Luigi Chieco-Bianchi
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D'Andrea, E., Tessarollo, L., Menin, C. et al. Tumours induced by Moloney murine sarcoma virus are clonal in rats, not clonal in mice. J Cancer Res Clin Oncol 118, 141–146 (1992). https://doi.org/10.1007/BF01187503

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  • DOI: https://doi.org/10.1007/BF01187503

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