Summary
The antiproliferative activity of the non-myelotoxic antitumour agent of plant origin, Thaliblastine, on two human glioma cell lines is described. Thaliblastine was added once one day following start of culture; proliferation was monitored over 7 days. The anti-proliferative activity of Thaliblastine was strongly dependent on concentration and time of incubation. The ID50 of Thaliblastine in T406 and GW27 glioma lines was 5.1 μg/ml and 8.2 μg/ml (7.0 μM and 11.2 μM), respectively.
Abbreviations
- TBL:
-
Thaliblastine
References
Allen L, Creaven P (1973) Inhibition of macromolecular biosynthesis in cultured L 1210 mouse leukemia cells by Thalicarpine (NSC-68075). Cancer Res 33:3112–3116
Arnaudov I, Georgiev G, Petrov M, Serbesova M (1986) The effect of the Bulgarian antineoplastic agent Thaliblastine in the treatment of patients with malignant lymphomas. 1st International Symposium on Clinical Pharmacology, Sofia. Abstract 13
Broder L, Carter S (1971) Thalicarpine (NSC-68075). Clinical Brochure. National Cancer Institute, Bethesda, USA
Christov I, Krusteva E (1983) Thaliblastine-potential plant antitumor agent. Phase I of clinical trial. Sovremenna Med 34:381–385
Christov I, Krusteva E (1985) Thaliblastine — a potential plant anti-tumor preparation. In: Eckhardt S, Kerpel-Fronius S (eds) CMEA — Chemotherapy Symposium, Akademiai Kiado, Budapest, pp 497–502
Christov I, Krusteva E, Ognyanova V, Tanev S (1986) Thaliblastine — the I–II phase clinical trial. 14th International Cancer Congress Budapest. Abstract 452
Creaven P, Allen L (1976) Thalicarpine (NSC-68075): plasma decay and urinary excretion in man. Cancer Treat Rep 60:69–75
Creaven P, Cohen M, Selawry D, Tejada F, Broder L (1975) Phase I study of Thalicarpine (NSC-68075), a plant alkaloid of novel structure. Cancer Chemother Rep 59:1001–1006
Deliconstantinos G, Ramantanis G, Todorov D (1983) Interaction of 99m Tc-labeled liposomes with Walker tumor cells in vitro. Liposome-mediated introduction of Thaliblastine into resistant Walker tumor cells. Gen Pharmacol 14:407–411
Fischer H, Zeller WJ, Schwechheimer K, Hutter K-J, Wowra B, Kunze S, Sturm V (1989) Establishment and characterization of human glioblastoma cell lines in vitro and their xenografts in nude mice. Onkologie 12:169–174
Freeman A, Fenstermacher J, Shapiro W, Kemshead J, Chasin M, Colvin O, Diksic M, Finley J, Hertler A, Levin V, Mayhew E, Poplack D, Shapiro J, Ushio Y (1990) Forbeck forum on improved drug delivery to brain tumours. Sel Cancer Therap 6:109–118
Ilarionova M, Maneva K, Todorov D (1980) Pathomorphological studies on animals bearing experimental tumors treated with Thaliblastine. C R Acad Bulg Sci 33:719–722
Kornblith P, Walker M (1988) Chemotherapy of malignant gliomas. J Neurosurg 68:1–17
Kupchan S, Chakravarti K, Yokoyoma N (1963) Thalicarpine, a new hypotensive alkaloid fromThalictrum dasycarpum. J Pharm Sci 52:985–988
Kupchan S, Liepa A, Kameswaran V, Sempuku K (1973) Total synthesis of the tumor-inhibitory alkaloid Thalicarpine. J Am Chem Soc 95:2995–3000
Mircheva J, Stoychkov J (1976) Effect of Thaliblastine on transplantable tumors in mice. Biomed Exp 25:280–281
Mollov N, Dutschewska H, Panov P (1964) Über die Alkaloide vonThalictrum minus L. ssp. elatum bulgarischer Herkunft. C R Acad Bulg Sci 17:709–712
Mollov N, Dutschewska H, Siljanovska K, Stojcev S (1968) Cytotoxic effect of alkaloids fromThalictrum minus ssp. elatum and their derivatives. C R Acad Bulg Sci 21:605–608
Philips B (1974) A simple, small scale cytotoxicity test, and its uses in drug metabolism studies. Biochem Pharmacol 23:131–138
Todorov D (1983) Pharmacological approaches to modifying the effects of some antitumor agents. Thesis D. Sc., Sofia
Todorov D (1988) Thaliblastine. Drugs Future 13:234–238
Todorov D, Damyanova M (1975) Investigations into the acute toxicity of the antitumor alkaloid Thaliblastine. C R Acad Bulg Sci 28:709–711
Todorov D, Deliconstantinos G (1982) Incorporation of the antitumor alkaloid Thaliblastine in liposomes enhances its cytotoxic activity in vitro. Experientia 38:857–858
Tomita M, Furukawa H, Lu S, Kupchan S (1965) The constitution of Thalicarpine. Tetrahedron Lett 48:4309–4316
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Todorov, D.K., Zeller, W.J. Antiproliferative activity of the non-myelotoxic antitumour agent of plant origin, Thaliblastine, on two human glioma cell lines. J Cancer Res Clin Oncol 118, 83–86 (1992). https://doi.org/10.1007/BF01187497
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DOI: https://doi.org/10.1007/BF01187497