Abstract
The level of norepinephrine in the extracellular space of the lateral hypothalamus was measured by means of intracerebral microdialysis in awake rats. The introduction of desipramine (10 Μmole) into the perfusing medium did not affect the basal level of norepinephrine, but increased the release of norepinephrine during local K+-stimulation. Neuroglycopenia created under the influence of 500 mg/kg of 2-deoxyglucose induced a threefold increase in the level of norepinephrine in the extracellular space of the hypothalamus, which achieved a maximal value in the first 40 min following the introduction of the substance, and thereafter gradually decreased to the basal level.
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Literature cited
A. I. Kuz'min, V. S. Shul'zhenko, O. S. Medvedev, and V. I. Kapel'ko, “The association between the breakdown of adenine nucleotides, the loss of catecholamines in the presence of myocardial ischemia and during the recovery of contractile function during reperfusion,”Byul. Vsesoyuz. Kardiol. Nauch. Tsentra AMN SSSR, No. 1, 75–82 (1987).
O. S. Medvedev, A. I. Kuz'min, G. Ya. Khulup, and O. B. Anosova, “2-Desoxyglucose as a tool for the analysis of the humoral component of the reaction of the cardiovascular system to stress,”Byull. Éksp. Biol. Med.,106, No. 2, 143–145 (1988).
A. M. Dart, A. Schomig, R. Dietz, et al., “Release of endogenous catecholamines in the ischemic myocardium of the rat. Part B: Effect of sympathetic nerve stimulation,”Circ. Res.,55, No. 5, 702–706 (1984).
G. Eisenhofer, T. G. Ropchak, R. W. Stull, et al., “Dihydroxyphenylglycol and intraneuronal metabolism of endogenous and exogenous norepinephrine in the rat vas deferens,”J. Pharmacol. Exp. Ther.,241, No. 2, 547–553 (1987).
B. Larsson, K. Svardsudd, L. Welin, et al., “Obesity, adipose tissue distribution and health — the study of men born in 1913,” in:Obesity in Europe 88, P. Bjorntorp and S. Rossner (eds.), John Libbey & Company Ltd., London, Paris (1989), pp. 49–54.
O. S. Medvedev, M. Deile, and P. Thoren, “2-deoxy-D-glucose-induced central glycopenia differentially influences renal and adrenal nerve activity in awake SHR rats,”Clin. Experim, Hypertension,A10, Suppl. 1, 375–381 (1988).
O. S. Medvedev, A. I. Kusmin, E. V. Oranovskaya, et al., “Cardiovascular correlates of metabolic stress elicited by 2-deoxy-D-glucose,” in:Stress: Neurochemical and Humoral Mechanisms, G. R. Van Loon, R. Kvetnansky, R. McCarty, and J. Axelrod (eds.), N. Y. (1989), pp. 691–703.
O. S. Medvedev, A. I. Kusmin, V. N. Selivanov, et al., “Pharmacological and physiological analysis of catecholamine secretion by the adrenal gland using in vivo microdialysis in the awake rat,” in:International Symposium on Microdialysis. Current Separations (Bioanalytical Systems), No. 3, (1989), p. 89.
Y. Oomura, “Contribution of endogenous substances to control of feeding,” in:Obesity in Europe 88, P. Bjorntorp and S. Rossner (eds.), John Libbey & Company Ltd., London, Paris (1989), pp. 101–108.
G. Paxinos and C. Watson,The Rat Brain in Stereotaxic Coordinates, Australia (1986).
G. M. Reaven, H. Ho, and B. B. Hoffman, “Somatostatin inhibition of fructose-induced hypertension,”Hypertension,14, No. 1, 117–120(1989).
N. E. Rowland and J. Carlton, “Dexfenfluramine: effects on food intake in various animal models,”Clin. Neuropharmacology,11, Suppl. l, S33-S50 (1988).
A. Schomig, A. M. Dart, R. Dietz, et al., “Release of endogenous catecholamines in the ischemic myocardium of the rat. Part A: Locally mediated release,”Circ. Res.,55, No. 5,689–701 (1984).
H. Shimizu and G. A. Bray, “Hypothalamic monoamines measured by microdialysis in rats treated with 2-deoxy-glucose or d-fenfluramine,”Physiol. Behavior,46, No. 5, 799–807 (1989).
G. Smythe, H. Grunstein, J. Bradshaw, et al, “Relationship between brain noradrenergic activity and blood glucose,”Nature (L.),308, 65–67 (1984).
L. H. Storlien, H. S. Grunstein, and G. A. Smythe, “Guanethidine blocks the 2-deoxy-D-glucose-induced hypothalamic noradrenergic drive to hyperglycemia,”Brain Res.,335, No. 1, 144–147 (1985).
C. L. Sun, N. B. Thoa, and I. J. Kopin, “Comparison of the effects of 2-deoxyglucose and immobilization on plasma levels of catecholamines and corticosterone in awake rats,”Endocrinology,105, No. 1, 306–311 (1979).
U. Ungerstedt, “Measurement of neurotransmitter release by intracranial dialysis,” in:Measurement of Neurotransmitter Release in Vivo, New York (1984), pp. 81–107.
K. Uvnas-Moberg, “Neuroendocrine regulation of hunger and satiety,” in:Obesity in Europe 88, P. Bjorntorp and S. Rossner (eds.), John Libbey & Company Ltd., London, Paris (1989), pp. 1–13.
S. Wallenstein, K. L. Zucker, and J. L. Fleiss, “Some statistical methods useful in circulation research,”Circ. Res.,47, No. 1, 1–9 (1980).
N. Yamaguchi, D. Lamoutagne, G. Boudreau, et al., “Effects of yohimbine and desipramine on cardiac noradrenaline release and ventricular arrhythmias during acute coronary artery occlusion and reperfusion in anesthetized dogs,”Can. J. Physiol. Pharmacol.,65, No. 11, 2244–2253 (1987).
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Translated from Fiziologicheskii Zhurnal SSSR imeni I. M. Sechenova, Vol. 77, No. 5, pp. 47–52, May, 1991.
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Kuz'min, A.I., Bogdanov, M.B. & Medvedev, O.S. A microdialysis investigation of the release of norepinephrine in the hypothalamus induced by 2-deoxyglucose in awake rats. Neurosci Behav Physiol 22, 436–439 (1992). https://doi.org/10.1007/BF01186639
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DOI: https://doi.org/10.1007/BF01186639