Conclusions
-
1.
Antibodies circulating in the blood, to proteins present in the nerve tissue of the rat — 10-40-4 from the rat brain, 14-3-2, and tubulin — prevent, the development of conditioned reactions in rats in a T-shaped maze. In this case the most specific effect (a change in the number of conditioned responses and the number of errors) is exhibited by antibodies to the neurospecific protein 10-40-4 from the rat brain.
-
2.
Antibodies circulating in the blood, to proteins that are not present in nerve tissue — 10-40-4 from human brain and bovine serum albumin — do not affect the development of conditioned responses in rats in a T-shaped maze.
-
3.
The presence of an effect of antibodies to the protein 10-40-4 from rat brain on the process of learning in rats confirms the permeability of the blood-brain barrier to these antibodies in immunized animals.
Similar content being viewed by others
Literature cited
V. V. Dergachev and O. N. Dolgov, “Inhibition of memory in rats by antibodies against the brains of irradiated rats,” Byul. Éksp. Biol. Med.,71, No. 64, 12 (1971).
S. D. Zaiko, N. I. Sokolova, T. P. Klyushnik, and G. Sh. Burbaeva, “Neurospecific proteins 14-3-2, 14-3-3, and 10-40-4: isolation and properties,” Biokhimiya,49, No. 3, 355 (1984).
B. I. Klement'ev, L. N. Grinkevich, T. S. Glushchenko, V. S. Repin, and L. Z. Pevzner, “Development of a conditioned reflex of passive avoidance and turnover of the proteins 14-3-2 and S-100 in the rat hippocampus,” Dokl. Akad. Nauk SSSR,221, No. 1, 243 (1975).
T. P. Klyushnik, G. Sh. Burbaeva, and O. C. Bashkirova, “Comparative immunochemical and physicochemical study of the neurospecific antigen 10-40-4 from the human and rat brain,” Zh. Évol. Biokhim. Fiziol.,20, No. 3, 257 (1984).
N. A. Nazarenko, “The quantitative reaction of complement fixation according to a 50% titer,” in: Laboratory Immunology [in Russian], Meditsina, Moscow (1967), p. 176.
V. Yu. Urbakh, Statistical Analysis in Biological and Medical Research [in Russian], Meditsina, Moscow (1975), p. 110.
E. D. Day, L. Rigsbee, R. Wilkins, and M. S. Mahaley, “Localization of antibrain radioantibodies in rat brain,” J. Immunol.,98, No. 1, 62 (1967).
R. G. Heath, S. M. Krupp, and G. S. Libjekist, “Schizophrenia as an immunologic disorder. Effects of antimonkey antibodies on brain function,” Arch. Gen. Psychiatr.,16, No. 1, 24 (1967).
S. A. Hoffman, D. N. Arbogast, T. T. Day, D. W. Shusarg, and R. J. Harbeck, “Permeability of the blood cerebrospinal fluid barrier during acute immune complex disease,” J. Immunol.,130, No. 4, 1695 (1983).
H. Hyden and P. W. Lange, “S-100 protein: correlation with behavior,” Proc. Natl. Acad. Sci. USA,64, No. 4, 1959 (1970).
H. Hyden, P. W. Lange, and V. Seifried, “Biochemical brain protein changes produced by selective breeding for learning in rats,” Brain Res.,61, No. 3, 446 (1973).
B. D. Jankovič, J. Horvat, K. Mitrovič, and M. Mastarica, “Rat brain lymphocyte antigen: characterization by rabbit antisera to rat brain tubulin and S-100 protein,” Immunochemistry,14, No. 1, 75 (1977).
S. E. Karpiak, M. Serokosz, and M. M. Rapport, “Effects of antisera to S-100 protein and to synaptic membrane fraction on maze performance and EEG,” Brain Res.,102, No. 2, 313 (1976).
L. Lim, C. Hall, T. Lenng, L. Mahadezan, and S. Whatley, “Neuron-specific enolase and creatine phosphokinase are protein components of rat brain synaptic plasma membranes,” J. Neurochem.,41, No. 4, 1177 (1983).
C. F. C. McPherson and R. P. N. Shek, “Effect of brain antibodies on learning and memory in rat,” Exptl. Neurol.,29, No. 1, 1 (1970).
L. Mihailovič and B. D. Janković, “Effect of anticerebral antibodies on electrical activity and behavior,” Neurosci. Res. Progr. Bull.,3, No. 1, 8 (1965).
N. S. Peres, F. Miller, and W. Palu, “The immunopathophysiological effects of chronic serum sickness on rat choroid plexus, ciliary process and renal gloineruli,” J. Neuropathol. Exper. Neurol.,36, No. 2, 726 (1977).
M. L. Shelanski, F. Gaskin, and C. R. Cantor, “Microtubule assembly in the absence of added nucleotides,” Proc. Natl. Acad. Sci. USA,70, No. 3, 765 (1973).
P. Sherline, C. K. Bodwin, and D. M. Kippnis, “A new colchicine binding assay for tubulin,” Anal. Biochem.,62, No. 2, 400 (1974).
C. Zomzely-Neurath and A. Keller, “The different forms of brain enolase: isolation, characterization, cell specificity and physiological significance,” in: Mechanisms, Regulation and Special Functions of Protein Synthesis in the Brain, North Holland Biomedical Press, Amsterdam, New York, Elsevier (1977), p. 279.
Author information
Authors and Affiliations
Additional information
Translated from Zhurnal Vysshei Nervnoi Deyatel'nosti imeni I. P. Pavlova, Vol. 35, No. 4, pp. 747–752, July–August, 1985.
Rights and permissions
About this article
Cite this article
Burbaeva, G.S., Kamenskii, A.A., Klyushnik, T.P. et al. Influence of immunization with neurospecific proteins and tubulin on learning in rats. Neurosci Behav Physiol 16, 384–388 (1986). https://doi.org/10.1007/BF01185368
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01185368