Abstract
Neuronal isolation of the rabbit cerebral hemisphere shifts the EEG spectrum in the direction of slower processes. Application of acetylcholine to the cortex brings about EEG activation and appearance of the theta rhythm. Initially serotonin application is accompanied by the appearance of theta rhythm periods; during subsequent administration of the drug these periods are gradually substituted by slow delta waves. Combined application of serotonin and acetylcholine to the isolated cortex brings about bursts of high amplitude activity, abruptly substituted by “silent” phases. In contrast to the intact cortex, where serotonin brought about prolonged and rhythmic alternation on the EEG of phases of high amplitude activity and of silent periods, in the isolated cortex the bursts of activity of about 1 min duration appeared only after application of acetylcholine to the serotonin-saturated cortex. Repeated phases of activation were either absent or of short duration and were rapidly extinguished.
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N. N. Agladze, “Spectral analysis of electroencephalograms of neuronally isolated rabbit cortex,” Fiziol. Zhurn. SSSR,68, No. 9, 1287 (1982).
N. N. Agladze, B. V. Bakharev, and M. N. Zhadin, “Spontaneous impulse activity of neurons of the isolated rabbit cerebral cortex,” Zhurn. Vyssh. Nervn. Deyat.,34, No. 3, 510 (1984).
A. R. Akopyan, B. N. Veprintsev, V. I. Il'in, and N. K. Chemeris, “Biochemical modulation of the functional state of choline receptors induced by serotonin as a possible mechanism of synaptic plasticity,” Dokl. Akad. Nauk SSSR, Ser. Biol.,259, No. 3, 753 (1981).
M. M. Bogoslovskii, M. M. Khananashvili, and É. G. Zarkeshev, “Neurophysiological characterization of the isolated cortical structures of the large hemispheres,” Uspekhi Fiziol. Nauk,4, No. 2, 55 (1973).
G. G. Gasanov, M. N. Zhadin, Z. G. Mamedov, and Z. A. Abdullaeva, “Oscillatory processes in the activity of cortical neurons by the action of serotonin,” Fiziol. Zhurn. SSSR,65, No. 9, 1257 (1979).
G. G. Gasanov, M. N. Zhadin, and Z. G. Mamedov, “Variations in the structure of background impulsation of a cortical neuron by the action of noradrenalin,” Fiziol. Zhurn. SSSR,66, No. 10, 1446 (1980).
E. A. Gromova, Emotional Memory and its Mechanisms [in Russian], Nauka, Moscow (1980).
M. N. Zhadins “Model of formation of a conditioned reflex and analysis of the functional significance of the electrographic correlates of training,” Zhurn. Vyssh. Nervn. Deyat.,27, No. 5, 949 (1977).
M. N. Zhadin, Theory of Rhythmic Processes in the Cerebral Cortex [in Russian], NTsBI Academy of Sciences of the USSR, Pushchino (1982).
M. N. Zhadin and B. V. Bakharev, “Model of the behavior of neurons under the action of biogenic amines,” Summaries of Reports of the First All-Union Biophysical Conference, Moscow (1982), Vol. 3, p. 33.
M. N. Zhadin, L. P. Yakupova, and I. O. Muradova, “Effect of stimulation of the raphe nuclei and the nuclei of the blue spot on the reticulocortically induced responses,” Summaries of reports of the Seventh All-Union Conference on the Electrophysiology of the CNS, Erevan (1980), p. 288.
A. Ya. Ivanov, “Action of serotonin on the membrane potential of neurons of the upper rabbit cervical ganglion,” in: Physiology and Biochemistry of Mediator Processes, Summaries of Reports of the Third All-Union Conference, Moscow (1982), p. 83.
D. A. Ignat'ev, “Change in the electrical activity of the rabbit cerebral cortex under the action of noradrenalin application,” Summaries of Reports of All-Union Symposium “Neurochemical Mechanisms of Memory Regulation,” Pushchino (1984), p. 60.
D. A. Ignat'ev and Z. G. Mamedov, “Action of serotonin on the electrical activity of rabbit cerebral cortex,” Zhurn. Vyssh. Nervn. Deyat.,33, No. 1, 129 (1983).
R. Yu. Il'yuchenok and M. A. Gilinskii, Construction and Mediators of Reticulo-cortical Connections, Nauka, Leningrad (1971).
S. N. Kozhechkin and S. D. Zhadina, “Microionophoretic study of the serotonin and acetylcholine on the activity of neurons of the rabbit sensorimotor cortex,” in: Cellular Mechanisms of Memory, NTsBI Academy of Sciences of the USSR, Pushchino (1973), p. 71.
M. M. Khananashvili, Neuronally Isolated Cortex [in Russian], Meditsina, Leningrad (1971).
A. Beandet and L. Descarries, “The monoamine innervation of rat cerebral cortex: synaptic; and nonsynaptic terminals,” Neuroscience,3, No. 10, 851 (1978).
T. Crow, J. Deakin, S. File, A. Longden, and S. Wendladt, “The locus coeruleus noradrenergic system — evidence against a role in attention, habituation, anxiety, and motor activity,” Brain Res.,155, No. 2, 249 (1978).
K. Dismukes, “New look at the aminergic nervous system,” Nature,269, No. 5629, 557 (1977).
S. Kety, “Brain catecholamines, affective states and memory,” in: The Chemistry of Mood, Motivation, and Memory, T. L. McGaugh, ed., New York (1972), p. 65.
K. Krnjevic, “Chemical nature of synaptic transmission in vertebrates,” Physiol. Rev.,54, No. 2, 418 (1974).
K. Krnjevic, K. Pumain, and L. Renand, “The mechanism of excitation by acetylcholine in the cerebral cortex,” J. Physiol. (Engl.),215, No. 2, 247 (1971).
B. Libet, H. Kobayashi, and T. Tanaka, “Synaptic coupling into the production and storage of a neuronal memory trace,” Nature,258, 5531, 155 (1975).
J. Olds and P. M. Milner, “Positive reinforcement produced by electrical stimulation of septal area and other regions of rat brain,” J. Compar. Physiol. Psychol.,47, No. 2, 419 (1954).
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Translated from Zhurnal Vysshei Nervnoi Deyatel'nosti imeni I. P. Pavlova, Vol. 35, No. 3, pp. 733–741, July–August, 1985.
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Ignat'ev, D.A., Agladze, N.N. & Zhadin, M.N. Effect of serotonin and acetylcholine on electrical activity of the isolated rabbit cortex. Neurosci Behav Physiol 16, 376–383 (1986). https://doi.org/10.1007/BF01185367
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DOI: https://doi.org/10.1007/BF01185367