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Male-sterile chicory cybrids obtained by intergeneric protoplast fusion

Theoretical and Applied Genetics volume 87, pages 347–352 (1993)Cite this article

Abstract

Male-sterile chicory plants were obtained by fusion of chicory mesophyll protoplasts and hypocotyl protoplasts derived from male-sterile sunflower plants. The protoplasts of both species were fused by the PEG method and the products were selected manually and cultivated at very low density in a liquid medium. Three to twenty percent of the heterokaryocytes divided and evolved into microcalli, then into calli where budding could be induced. The mitochondrial genome of ten male-sterile or totally sterile plants was studied. Restriction endonuclease profiles of mitochondrial DNA and molecular hybridization with specific genes of the mitochondrial genome used as probes indicated that mitochondrial DNA rearrangement had occurred between sunflower and chicory and the intensity of the rearrangements correlated with the degree of sterility of the different plants.

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Affiliations

  1. Laboratoire de Physiologie Cellulaire et Morphogenèse Végétales, Université des Sciences et Technologies de Lille-Flandres-Artois, 59655, Villeneuve d'Ascq cedex, France

    C. Rambaud, J. Dubois & J. Vasseur

Authors
  1. C. Rambaud
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  2. J. Dubois
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  3. J. Vasseur
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Communicated by G. Melchers

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Rambaud, C., Dubois, J. & Vasseur, J. Male-sterile chicory cybrids obtained by intergeneric protoplast fusion. Theoret. Appl. Genetics 87, 347–352 (1993). https://doi.org/10.1007/BF01184922

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  • DOI: https://doi.org/10.1007/BF01184922

Key words

  • Cichorium intybus
  • Protoplast fusion
  • Cytoplasmic male sterility
  • Mitochondrial DNA