Abstract
Histological changes are shown of ossification induced by a simultaneous exposure to zinc and cadmium or to zinc and copper using embryonic chick femur in a culture system. Cadmium caused an atrophic change of the osseous tissue in the absence of zinc but caused an osteomalacic change with a partial degenerative change in the presence of zinc after a 6-day culture. Copper caused an atrophic change in the absence or presence of zinc. These observations were partly supported by the fact that the diaphysial calcium content was significantly decreased by zinc alone, and the decrease was unaffected by cadmium or copper. Zinc significantly decreased cadmium accumulation but not copper accumulation in the diaphysis. Thus, in spite of the inhibitory effect on calcification, zinc prevented a decrease in bone matrix formation caused by cadmium but not that by copper. Exposure of chick femur culture to zinc and cadmium induced changes consistent with osteomalacia, i.e., decreased mineralization of bone, with or without suppression of matrix formation. Exposure to zinc and copper, however, induced changes consistent with osteoporosis, i.e., decreased mineralization and matrix formation.
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Endo H (1974) Cartilage matrix metabolism of chondrocytes changing through their life span and its possible hormonal control, as demonstrated by tissue culture studies. Connect Tissue 6:139–148
Hagino N, Yoshioka K (1961) Study on a case of Itai-itai disease. J Jpn Orthop Assoc 35:812–815
Itokawa Y, Abe T, Tanaka S (1973) Bone changes in experimental chronic cadmium poisoning. Arch Environ Health 26:241–244
Kaji T, Kawatani R, Takata M, Hoshino T, Miyahara T, Kozuka H, Koizumi F (1988a) The effects of cadmium, copper or zinc on formation of embryonic chick bone in tissue culture. Toxicology 50:303–316
Kaji T, Takata M, Hoshino T, Miyahara T, Kozuka H, Kurashige Y, Koizumi F (1988b) Role of zinc in protection against cadmium-induced toxicity in formation of embryonic chick bone in tissue culture. Toxicol Lett 44:219–227
Kaji T, Yamada H, Hoshino T, Miyahara T, Kozuka H, Naruse Y (1986) A possible mechanism of cadmium-copper interaction in embryonic chick bone in tissue culture. Toxicol Appl Pharmacol 86:243–252
Kimura M, Otaki N, Yoshiki S, Suzuki M, Horiuchi N, Suda T (1974) The isolation of metallothionein and its protective role in cadmium poisoning. Arch Biochem Biophys 165:340–348
Kitagawa M, Miwa A, Murai Y (1983) Pathological study on twenty seven autopsied cases with Itai-itai disease in Toyama Prefecture. Reports on Environmental Health 49:140–145
Kobashi K, Nakai N, Hase J, Miyahara T, Kozuka H, Fujii M (1978) Chemical forms of cadmium in cadmium-polluted rice. I. Binding properties of gluterin-cadmium complex. Eisei Kagaku 24:314–321
Miyahara T, Kozuka H (1985) The direct effect of cadmium on bone metabolism and interaction between cadmium and zinc or copper in tissue culture. Eisei Kagaku 31:59–71
Miyahara T, Oh-e Y, Takaine E, Kozuka H (1983) Interaction between cadmium and zinc, copper or lead in relation to the collagen and mineral content of embryonic chick bone in tissue culture. Toxicol Appl Pharmacol 67:41–48
Miyahara T, Sugiyama S, Kaji T, Yamashita R, Oh-e Y, Kurano T, Kozuka H (1984) Interaction between cadmium and copper in relation to the collagen metabolism of embryonic chick bone in tissue culture. Toxicol Appl Pharmacol 75:479–484
Miyahara T, Yamada H, Ando R, Nemoto S, Kaji T, Mori M, Kozuka H, Itoh N, Sudo H (1986) The effects of cadmium on a clonal osteogenetic cell, MC3T3-E1: Inhibition of calcification and induction of metallothionein-like protein by cadmium. Toxicol Lett 32:19–27
Nogawa K (1983) Itai-itai disease and follow-up studies. In: Nriagu JO (ed) Cadmium in the environment, Part II: Health effects. John Wiley & Sons, New York, pp 1–37
Sakai T, Miyahara T, Sanei K, Nomura N, Takayanagi N (1975) Hygienic chemical studies on environmental pollution. I. Effect of cadmium on chick-embryo tibia in tissue culture. Eisei Kagaku 21:35–41
Schenk KR, Olar AJ and Herrman W (1984) Preparation of calcified tissues for light microscopy. In: Dickson GR (ed) Method of calcified tissue preparation. Elsevier, Amsterdam, pp 1–56
Suzuki KT (1981) Heavy metals and metallothionein. J Synth Org Chem Japan 39:1073–1082
Takeuchi T, Nakano Y, Aoki A, Ohmori S, Tsukatani T (1986) Comparisons of elemental concentrations in hair of the inhabitants of heavy metal polluted areas with those of normal Japanese. I. Concentration difference and correlation coefficient. Annu Rep Res Reactor Inst, Kyoto Univ 19:89–98
Tenenbaum HC, Heersch JNM (1982) Differentiation of osteoblasts and formation of mineralized bonein vitro. Calcif Tissue Int 33:76–79
Yoshiki S, Yanagishita T, Kimura M, Otaki N, Suzuki M, Suga T (1975) Bone and kidney lesions in experimental cadmium intoxication. Arch Environ Health 30:559–562
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Kaji, T., Takata, M., Miyahara, T. et al. Interaction of zinc with cadmium and copper on ossification of embryonic chick bone in tissue culture. Arch. Environ. Contam. Toxicol. 19, 653–656 (1990). https://doi.org/10.1007/BF01183980
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DOI: https://doi.org/10.1007/BF01183980