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Residues of pentachlorophenol and other chlorinated contaminants in human tissues: Analysis by electron capture gas chromatography and electron capture negative ion mass spectrometry

Archives of Environmental Contamination and Toxicology volume 21, pages 596–606 (1991)Cite this article

Abstract

Samples of human tissues including testes, kidneys, prostate glands, livers, and adipose tissues removed at autopsy were analyzed for pentachlorophenol (PCP) and nonachloro-2-phenoxyphenol (NCPP); the fat samples were also analyzed for other chlorinated contaminants. Electron capture gas chromatography was used to quantitate the residues after isolation and cleanup. Identity of the residues was confirmed by electron capture negative ion mass spectrometry. All tissues analyzed for PCP tested positive with a range from 0.007 ppm (μg/g) in subcutaneous fat to 4.14 ppm in testis. Residues of NCPP were much lower, ranging from levels below the detection limit to 0.59 ppm in testis. The highest average residues, based on the lipid content of the tissue, of PCP (1.09 ppm) and of NCPP (0.19 ppm) were found in testis followed by kidney (0.95 ppm PCP), prostate (0.84 ppm PCP), and liver (0.59 ppm PCP). Residues of these chemicals in the adipose tissues were on the average about 40 times lower than the residues in the non-fatty tissues. Other chlorinated contaminants found in the subcutaneous fat included hexa-, hepta-, and octa-chlorodibenzo-p-dioxins at sub-ppb levels and DDE averaging 2.47 ppm.

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References

  • Ahlborg UG, Lindgren JE, Mercier M (1974) Metabolism of pentachlorophenol. Arch Toxicol 32:271–281

    PubMed  Google Scholar 

  • Arsenault RD (1976) Pentachlorophenol and contained chlorinated dibenzodioxins in the environment. Proceedings of the 72nd Annual Meeting of the American Wood Preservers' Association, pp 122–148

  • Atuma SS, Okor DI (1985) Gas Chromatographic determination of pentachlorophenol in human blood and urine. Bull Environ Contam Toxicol 35:406–410

    PubMed  Google Scholar 

  • Bevenue A, Beckman H (1967) Pentachlorophenol: A discussion of its properties and its occurrence as a residue in human and animal tissues. Residue Rev 19:83–134

    PubMed  Google Scholar 

  • Bevenue A, Haley TJ, Klemmer HW (1967) A note on the effects of a temporary exposure of an individual to pentachlorophenol. Bull Environ Contam Toxicol 2:293–296

    Google Scholar 

  • Blaser WW, Bredgeweg RA, Shadoff LA, Stehl RH (1976) Determination of chlorinated dibenzo-p-dioxins in pentachlorophenol by gas chromatography-mass spectrometry. Anal Chem 48:984–986

    Google Scholar 

  • Braun WH, Blau GE, Chenoweth MB (1979) The metabolism and pharmacokinetics of pentachlorophenol in man and a comparison with the rat and monkey. Dev Toxicol Environ Sci 4:289–296

    Google Scholar 

  • Buhler DR, Rasmusson ME, Nakaue HS (1973) Occurrence of hexachlorophene and pentachlorophenol in sewage and water. Environ Sci Technol 7:929–934

    Google Scholar 

  • Buser H-R (1975) Polychlorinated dibenzo-p-dioxins, separation and identification of isomers by gas chromatography-mass spectrometry. J Chromatogr 114:95–108

    PubMed  Google Scholar 

  • — (1976) High-resolution gas chromatography of polychlorinated dibenzo-p-dioxins and dibenzofurans. Anal Chem 48:1553–1557

    Google Scholar 

  • Buser H-R, Bosshardt HP (1976) Determination of polychlorinated dibenzo-p-dioxins and dibenzofurans in commercial pentachlorophenols by combined gas chromatography-mass spectrometry. J Assoc Offic Anal Chem 59:562–569

    Google Scholar 

  • Casarett LJ, Bevenue A, Yauger WL Jr, Whalen SA (1969) Observations on pentachlorophenol in human blood and urine. Am Ind Hyg Assoc J 30:360–366

    PubMed  Google Scholar 

  • Cleveland L, Buckler DR, Mayer FL, Branson DR (1982) Toxicity of three preparations of pentachlorophenol to fathead minnows-a comparative study. Environ Toxicol Chem 1:205–212

    Google Scholar 

  • Cline RE, Hill RH Jr, Phillips DL, Needham LL (1989) Pentachlorophenol measurements in body fluids of people in log homes and workplaces. Arch Environ Contam Toxicol 18:475–481

    PubMed  Google Scholar 

  • Courtney KD (1979) Hexachlorobenzene (HCB): A review. Environ Res 20:225–266

    PubMed  Google Scholar 

  • Crosby DG (1981) Environmental chemistry of pentachlorophenol. Pure Appl Chem 53:1051–1080

    Google Scholar 

  • Deinzer M, Griffin D, Miller T, Skinner R (1979a) Identification of octachlorophenoxydiphenyl ethers in technical pentachlorophenol. Biomed Mass Spectrom 6:301–304

    Google Scholar 

  • Deinzer M, Miller T, Griffin D, Lamberton J, Arbogast B, Inman R (1979b) Isolation and identification of suspected toxicants in pentachlorophenol. Division of Environmental Chemistry, paper 309, 177th National American Chemical Society Meeting, Honolulu, Hawaii. American Chemical Society, Washington, DC, Vol 19, p 768

    Google Scholar 

  • Dougherty RC, Piotrowska K (1976) Screening by negative chemical ionization mass spectrometry for environmental contamination with toxic residues: Application to human urines. Proc Natl Acad Sci USA 73:1777–1781

    PubMed  Google Scholar 

  • Dougherty RC (1978) Human exposure to pentachlorophenol. In: Rao KR (ed) Pentachlorophenol: Chemistry, pharmacology, and environmental toxicology. Plenum Press, NY, pp 352–361

    Google Scholar 

  • Dougherty RC, Whitaker MJ, Smith LM, Stalling DL, Kuehl DW (1980) Negative chemical ionization studies of human and food chain contamination with xenobiotic chemicals. Environ Health Perspect 36:113–118

    Google Scholar 

  • Fahring R, Nilsson C-A, Rappe C (1978) Genetic activity of chlorophenols and chlorophenol impurities. In: Rao KR (ed) Pentachlorophenol: Chemistry, pharmacology, and environmental toxicology. Plenum Press, NY, pp 325–338

    Google Scholar 

  • Firestone D, Ress J, Brown NL, Barron RP, Damico JN (1972) Determination of polychlorodibenzo-p-dioxins and selected compounds in commercial chlorophenols. J Assoc Offic Anal Chem 55:85–92

    Google Scholar 

  • Firestone D, Clower M Jr, Borsetti AP, Teske RH, Long PE (1979) Polychlorodibenzo-p-dioxin and pentachlorophenol residues in milk and blood of cows fed technical pentachlorophenol. J Agric Food Chem 27:1171–1177

    PubMed  Google Scholar 

  • Geyer HJ, Scheunert I, Korte F (1987a) Correlation between the bioconcentration potential of organic environmental chemicals in humans and theirn-octanol/water partition coefficients. Chemosphere 16:239–252

    Google Scholar 

  • — (1987b) Distribution and bioconcentration potential of the environmental chemical pentachlorophenol (PCP) in different tissues of humans. Chemosphere 16:887–899

    Google Scholar 

  • Goldstein JA, Friesen M, Linder RH, Hickman P, Hass JR, Bergman H (1977) Effect of pentachlorophenol on hepatic drug metabolizing enzymes and porphyria related to contamination with chlorinated dibenzo-p-dioxins and dibenzofurans. Biochem Pharmacol 26:1549–1557

    PubMed  Google Scholar 

  • Grimm H-G, Löwer M, Valentin H (1984) Untersuchungen zur normalen Pentachlorphenol-Konzentration in menschlichen Organen. In: Umweltforschungsplan des Bundesministers des Innern. Forschungbericht 10704007. Pentachlorphenol als Modellfall einer Umweltchemikalie. Umweltbundesamt, Berlin

    Google Scholar 

  • Hattemer-Frey HA, Travis CC (1989) Pentachlorophenol: Environmental partitioning and human exposure. Arch Environ Contam Toxicol 18:482–489

    PubMed  Google Scholar 

  • Hedtke SF, West CW, Allen KN, Norberg-King TJ, Mount DI (1986) Toxicity of PCP to aquatic organisms under naturally varying and controlled environment conditions. Environ Toxicol Chem 5:531–542

    Google Scholar 

  • Hinkle DK (1973) Fetotoxic effects of pentachlorophenol in the golden Syrian hamster. Toxicol Appl Pharmacol 25:455

    Google Scholar 

  • International Programme on Chemical Safety (1987) Environmental Health Criteria 71. Pentachlorophenol. World Health Organization, Geneva, p 43

    Google Scholar 

  • Jensen S, Renberg L (1972) Contaminants in pentachlorophenol: Chlorinated dioxins and predioxins (chlorinated hydroxydiphenyl ethers). Ambio 1:62–65

    Google Scholar 

  • Jensen AA (1983) Chemical contaminants in human milk. Residue Rev 89:1–128

    PubMed  Google Scholar 

  • Johnson RL, Gehring PJ, Kociba RJ, Schwetz BA (1973) Chlorinated dibenzodioxins and pentachlorophenol. Environ Health Perspect 5:171–175

    PubMed  Google Scholar 

  • Johnson RD, Manske DD (1977) Pesticides in food and feed. Pesticide and other chemical residues in total diet samples (XI). Pestic Monitor J 11:116–131

    Google Scholar 

  • Kaufman DD (1978) Degradation of pentachlorophenol in soil, and by soil microorganisms. In: Rao KR (ed) Pentachlorophenol: Chemistry, pharmacology, and environmental toxicology. Plenum Press, NY, pp 27–39

    Google Scholar 

  • Kerkvliet NI, Baecher-Steppan L, Claycomb AT, Craig AM, Sheggeby GG (1982) Immunotoxicity of technical pentachlorophenol (PCP-T): Depressed humoral immune response to T-dependent and T-independent antigen stimulation in PCP-T exposed mice. Fundam App Toxicol 2:90–99

    Google Scholar 

  • Klemmer HW, Wong L, Sato MM, Reichert EL, Korsak RJ, Rashad MN (1980) Clinical findings in workers exposed to pentachlorophenol. Arch Environ Contam Toxicol 9:715–725

    PubMed  Google Scholar 

  • Koss G, Koransky W (1978) Pentachlorophenol in different species of vertebrates after administration of hexachlorobenzene and pentachlorobenzene. In: Rao KR (ed) Pentachlorophenol: Chemistry, pharmacology, and environmental toxicology. Plenum Press, NY, pp 131–137

    Google Scholar 

  • Kutz FW, Murphy RS, Strassman SC (1978) Survey of pesticide residues and their metabolites in urine from the general population. In: Rao KR (ed) Pentachlorophenol: Chemistry, pharmacology, and environmental toxicology. Plenum Press, NY, pp 363–369

    Google Scholar 

  • Kutz FW (1983) Chemical exposure monitoring. Residue Rev 85:277–292

    PubMed  Google Scholar 

  • Lamberton J, Griffin D, Arbogast B, Inman R, Deinzer M (1979) The determination of polychlorodibenzo-p-dioxins in pentachlorophenol and wood treatment solutions. Am Ind Hyg Assoc J 40:816–821

    PubMed  Google Scholar 

  • Lu P-Y, Metcalf RL, Cole LK (1978) The environmental fate of14C-pentachlorophenol in laboratory model ecosystems. In: Rao KR (ed) Pentachlorophenol: Chemistry, pharmacology, and environmental toxicology. Plenum Press, NY, pp 53–63

    Google Scholar 

  • McConnell EE, Moore JA, Gupta BN, Rakes AH, Luster MI, Goldstein JA, Haseman JK, Parker CE (1980) The chronic toxicity of technical and analytical pentachlorophenol in cattle. I. Clinicopathology. Toxicol Appl Pharmacol 52:468–490

    PubMed  Google Scholar 

  • Mieure JP, Hicks O, Kaley RC, Michael PR (1977) Determination of tracer amounts of chlorodibenzo-p-dioxins and chlorodibenzofurans in technical grade pentachlorophenol. J Chromatogr Sci 15:275–277

    Google Scholar 

  • Nilsson C-A, Norström A, Andersson K, Rappe C (1978) Impurities in commercial products related to pentachlorophenol. In: Rao KR (ed) Pentachlorophenol: Chemistry, pharmacology and environmental toxicology. Plenum Press, NY, pp 313–324

    Google Scholar 

  • Norén K (1988) Changes in the levels of organochlorine pesticides, polychlorinated biphenyls, dibenzo-p-dioxins and dibenzofurans in human milk from Stockholm, 1972–1985. Chemosphere 17:39–49

    Google Scholar 

  • Ohe T (1979) Pentachlorophenol residues in human adipose tissue. Bull Environ Contam Toxicol 22:287–292

    PubMed  Google Scholar 

  • Parker CE, Jones WA, Matthews HB, McConnell EE, Hass JR (1980) The chronic toxicity of technical and analytical pentachlorophenol in cattle. II. Chemical analysis of tissues. Toxicol Appl Pharmacol 55:359–369

    PubMed  Google Scholar 

  • Plimmer JR, Ruth JM, Woolson EA (1973) Mass spectrometric identification of the hepta- and octa-chlorinated dibenzo-p-dioxins and dibenzofurans in technical pentachlorophenol. J Agric Food Chem 21:90–93

    PubMed  Google Scholar 

  • Rappe C, Nilsson C-A (1972) An artifact in the gas Chromatographic determination of impurities in pentachlorophenol. J Chromatogr 67:247–253

    PubMed  Google Scholar 

  • Ryan JJ, Lizotte R, Sakuma T, Mori B (1985) Chlorinated dibenzo-p-dioxins, chlorinated dibenzofurans, and pentachlorophenol in Canadian chicken and pork samples. J Agric Food Chem 33:1021–1026

    Google Scholar 

  • Schwetz BA, Keeler PA, Gehring PJ (1974) The effect of purified and commercial grade pentachlorophenol on rat embryonal and fetal development. Toxicol Appl Pharmacol 28:151–161

    PubMed  Google Scholar 

  • Schwetz BA, Quast JF, Keeler PA, Humiston CG, Kociba RJ (1978) Results of two-year toxicity and reproduction studies on pentachlorophenol in rats. In: Rao KR (ed) Pentachlorophenol: Chemistry, pharmacology, and environmental toxicology. Plenum Press, NY, pp 301–309

    Google Scholar 

  • Shafik TM (1973) The determination of pentachlorophenol and hexachlorophene in human adipose tissue. Bull Environ Contam Toxicol 10:57–63

    Google Scholar 

  • Spindler M (1983) DDT: Health aspects in relation to man and risk/benefit assessment based thereupon. Residue Rev 90:1–34.

    PubMed  Google Scholar 

  • Stanley JS (1986) EPA Broad Scan Analysis of the FY 82 National Human Adipose Tissue Survey Specimens. December 31, 1986

  • Uhl S, Schmid P, Schlatter C (1986) Pharmacokinetics of pentachlorophenol in man. Arch Toxicol 58:182–186

    PubMed  Google Scholar 

  • Villanueva EC, Burse VW, Jennings RW (1973) Chlorodibenzo-p-dioxin contamination of two commercially available pentachlorophenols. J Agric Food Chem 21:739–740

    PubMed  Google Scholar 

  • Villanueva EC, Jennings RW, Burse VW, Kimbrough RD (1975) A comparison of analytical methods for chlorodibenzo-p-dioxins in pentachlorophenol. J Agric Food Chem 23:1089–1091

    PubMed  Google Scholar 

  • Williams PL (1982) Pentachlorophenol, an assessment of the occupational hazard. Am Ind Hyg Assoc J 43:799–810

    PubMed  Google Scholar 

  • Woolson EA, Thomas RF, Ensor PDJ (1972) Survey of polychlorodibenzo-p-dioxin content in selected pesticides. J Agric Food Chem 20:351–354

    PubMed  Google Scholar 

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Affiliations

  1. Department of Agricultural Chemistry, Oregon State University, 97331, Corvallis, Oregon, USA

    Sheldon L. Wagner, Lucia R. Durand, Roderick D. Inman, Ulo Kiigemagi & Max L. Deinzer

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  1. Sheldon L. Wagner
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  2. Lucia R. Durand
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  3. Roderick D. Inman
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  4. Ulo Kiigemagi
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  5. Max L. Deinzer
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Technical Paper No. 9586, Oregon Agricultural Experiment Station.

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Wagner, S.L., Durand, L.R., Inman, R.D. et al. Residues of pentachlorophenol and other chlorinated contaminants in human tissues: Analysis by electron capture gas chromatography and electron capture negative ion mass spectrometry. Arch. Environ. Contam. Toxicol. 21, 596–606 (1991). https://doi.org/10.1007/BF01183883

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  • DOI: https://doi.org/10.1007/BF01183883

Keywords

  • Adipose Tissue
  • Chlorinate
  • Lipid Content
  • Human Tissue
  • Electron Capture