Abstract
When the chromosome aberrations induced in human leukocytes in culture with daunomycin (Dm) (0.015 μg/ml, with or without added arginine) are analyzed with respect to sites of breakage and reunion, non-randomness is observed, both between and within chromosomes. These results suggest either a site-specificity of action of the drug or site-specific sensitivity or both. Superficially, the data give an indication of the phenomenon of somatic crossing-over having occurred. However, if chromosome breakage and reunions of broken ends are considered as two events, independent of each other, the frequency of exchanges between homologues and apparent homologues is lower than the expected frequency in cases of groups involving chromosomes 6–12/X and 13/15 and almost equal to the expected frequency in cases of chromosomes 1, 2 and 3.
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References
Auerbach, C.: The chemical production of mutations. Science158, 1141–1147 (1967).
Bhuyan, B. K., Smith, C. G.: Differential interaction of Nogalomycin with DNA of varying base composition. Proc. nat. Acad. Sci. (Wash.)54, 566–572 (1965).
Chicago Conference: Standardization in human cytogenetics. Birth defects II (edt. D. Bergsma), p. 21. U.S.A.: National Foundation — March of Dimes 1966.
Cohen, M. M.: The specific effects of streptonigrin activity on human chromosomes in culture. Cytogenetics2, 271–279 (1963).
—, Shaw, M. W.: Effects of mitomycin C on human chromosomes. J. Cell Biol.23, 386–395 (1964).
— —, Craig, A. P.: The effects of streptonigrin on cultured human leukocytes. Proc. nat. Acad. Sci. (Wash.)50, 16–24 (1963).
German, J.: Cytological evidence for crossing overin vitro in human lymphoid cells. Science144, 298–301 (1964).
Goldberg, I. H.: Mode of action of antibiotics. II. Drugs affecting nucleic acids and protein synthesis. Amer. J. Med.39, 722–752 (1965).
Grunberg, H.: The case of somatic crossing-over in the mouse. Genet. Res.7, 58–75 (1966).
Holliday, R.: The induction of mitotic recombination by mitomycin C inUstilago andSaccharomyces. Genetics50, 323–335 (1964).
Hsu, T. C., Schmidt, W., Stubblefield, E., in: Role of chromosomes in development, (edit. M. Locke). Proc. Soc. Study Growth and Develop.23, 83–112 (1964).
—, Somers, C. E.: Effect of 5-bromodeoxyuridine on mammalian chromosomes. Proc. nat. Acad. Sci. (Wash.)47, 396–403 (1961).
Moorhead, P. S., Nowell, P. C., Mellman, W. F., Battips, D. M., Hungerford, D. A.: Chromosome preparations of leukocyte cultures from human peripheral blood. Exp. Cell Res.20, 613–616 (1960).
Nowell, P. C.: Mitotic inhibition and chromosome damage by mitomycin in human leukocyte cultures. Exp. Cell Res.33, 445–449 (1964).
Ostertag, W., Kersten, W.: The action of proflavin and actinomycin D in causing chromatid breakage in human cells. Exp. Cell Res.39, 296–301 (1965).
Pontecorvo, G.: Trends in genetic analysis, p. 145. New York:Columbia University Press 1958.
Rao, R. N., Natarajan, A. T.: Somatic association in relation to chemically induced chromosome aberrations inVicia faba. Genetics57, 821–835 (1967).
Reich, E.: Actinomycin — Correlation of structure and function of its complexes with purines and DNA. Science143, 684–689 (1964).
Serebovsky, A. S.: “Somatic segregation” in domestic fowl. J. Genet.16, 33–42 (1925).
Shaw, M. W., Cohen, M. M.: Chromosome exchanges in human leukocytes induced by mitomycin C. Genetics51, 181–190 (1965).
Snedecor, G. W.: Statistical methods. Ames: Iowa State College Press 1956.
Somers, C. E., Hsu, T. C.: Chromosome damage induced by hydroxylamine in mammalian cells. Proc. nat. Acad. Sci. (Wash.)48, 937–943 (1962).
Stern, C.: Somatic crossing-over and segregation inDrosophila melanogaster. Genetics21, 625–630 (1936).
Tan, C., Tasaka, H., Yu, K. P., Murphy, M. L., Karnofsky, D. A.: Daunomycin, an antitumor antibiotic, in the treatment of neoplastic disease; clinical evaluation with special reference to childhood leukemia. Cancer (Philad.)20, 333–353 (1967).
Vig, B. K., Kontras, S. B., Aubele, A.: Sensitivity of G1 phase of mitotic cell cycle to chromosome aberrations induced by daunomycin. Mutation Res.7, 91–97 (1969).
— —, Paddock, E. F.:3H-thymidine induced chromosome aberrations in human leukocytes. Cytogenetics7, 189–195 (1968).
— — —, Samuels, L. D.: Daunomycin induced chromosome aberrations and the influence of arginine in modifying the effect of the drug. Mutation Res.5, 278–287 (1968).
— —, Samuels, L. D.: Chromosome aberrations induced by daunomycin in human leukocyte cultures with the apparent synergistic effect of arginine. Experientia (Basel)24, 271–273 (1968).
—, Paddock, E. F.: Alteration of leaf spot frequencies inGlycine max (L.) Merrill by mitomycin C. J. Hered.59, 225–229 (1968).
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Vig, B.K., Samuels, L.D. & Kontras, S.B. Specificity of daunomycin in causing chromosome aberrations in human leukocytes. Chromosoma 29, 62–73 (1970). https://doi.org/10.1007/BF01183661
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DOI: https://doi.org/10.1007/BF01183661