Abstract
AdultOncopeltus fasciatus males were irradiated with 9000 R of X-rays, and crossed to untreated females. Fertility was reduced to 4.1%. F1 males and F2 and F3 males and females were outcrossed to untreated partners. All F1 males were partially or totally sterile and a significant number of F2 and F3 males and females had reduced fertility. The fertility of each generation was higher than the preceding one, even though the progeny studied in the 3rd generation were selected mostly from low-fertility lines. Cytogenetic studies showed that complex chromosome rearrangements and fragments were transmitted to each generation and were severe enough to account for reduced fertility. — The transmission of complex chromosome rearrangements and fragments for 3 generations of outcrossing correlates with the persistence of sterility in this species possessing holokinetic chromosomes. — Over half the inviable embryos derived from irradiated sperm from P1 males died in the early stages of development. The inviable embryos produced in later generations died in much later stages of development. — A stable rearrangement of a Y-chromosome fragment translocated to an autosome was isolated from a single F1 male. This rearrangement was transmitted to all F2 and F3 sons. Fertility of the males of this line was reduced to about 75–80%.
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Bauer, H.: Die kinetische Organisation der Lepidopteren-Chromosomen. Chromosoma (Berl.)22, 102–126 (1967).
Buck, R. C.: Mitosis and meiosis inRhodnius prolixus: The fine structure of the spindle and diffuse kinetochore. J. Ultrastruct. Res.18, 489–501 (1968).
Burnham, C. R.: Discussions in Cytogenetics. Interchanges, p. 66–116 (chap. 4). Minneapolis, Minn.: Burgess Publishing Co. 1962.
Castro, D. de, Noronha-Wagner, M., Camara, A.: Two X-ray induced translocations inLuzula purpurea. Genetica Iberia6, 3–18 (1954).
Cogburn, R. R., Tilton, E. W., Burkholder, W. E.: Gross effects of gamma radiation on the Indian-meal moth and the Angoumis grain moth. J. econ. Entomol.61, 477–483 (1966).
Dikshith, T. S. S.: Studies on the cytology of coccids. II. Cytology ofRastrococcus iceroyides (Green) (Pseudococcidae-Coccoidea). Cytologia (Tokyo)31, 117–124 (1966).
Federley, H.: Zytologische Untersuchungen an Mischlingen der GattungDicranura B. (Lepidoptera). Hereditas (Lund)29, 205–254 (1943).
Flint, H. M., Kressin, E. L.: Transfer of sperm by irradiatedHeliothis virescens (Lepidoptera: Noctuidae) and relationship to fecundity. Can. Entomol.101, 500–507 (1969).
Halkka, O.: Chromosome studies on theHemiptera Homoptera Auchenorrhyncha. Ann. Acad. Sci. fenn. A IV43, 1–71 (1959).
—: X-ray induced changes in the chromosomes ofLimotettix (Homoptera). Chromosoma (Berl.)16, 186–191 (1965).
Helenius, O.: The mode of bivalent orientation in theHemiptera. Hereditas (Lund)38, 420–24 (1952).
Hughes-Schrader, S.: Cytology of Coccids (Coccoidea-Homoptera). Advanc. Genet.2, 127–203 (1948).
—, Ris, H.: The diffuse spindle fiber attachment of coccids, verified by the mitotic behavior of induced chromosome fragments. J. exp. Zool.87, 429–456 (1941).
—, Schrader, F.: The kinetochore of theHemiptera. Chromosoma (Berl.)12, 327–350 (1961).
LaChance, L. E.: The induction of dominant lethal mutations in insects by ionizing radiation and chemicals—as related to the sterile-male technique of insect control. In: Genetics of insect vectors of disease (J. W. Wright and R. Pal, eds.) (chap. 21, p. 617–650). Amsterdam: Elsevier 1967.
—, Degrugillier, M.: Chromosomal fragments transmitted through three generations inOncopeltus (Hemiptera), Science166, 236–236 (1969).
—, Schmidt, C. H., Bushland, R. C.: Radiation-induced sterilization. In: Pest control: Biological, physical, and selected chemical methods (W. W. Kilgore and R. L. Doutt, eds.) (chap. 4), p. 147–196. New York: Academic Press 1967.
Lima-de-Faria, A.: Genetics, origin and evolution of the kinetochore. Hereditas (Lund)35, 422–444 (1949).
Nordenskiöld, H.: Changes in somatic chromosome patterns in root tips of X-raytreatedLuzula. Kungl. Lantbrukshogskol. Ann.23, 273–289 (1957).
—: A study of meiosis in the progeny of X-irradiatedLuzula purpurea. Hereditas (Lund)49, 33–47 (1963).
North, D. T.: Radiation-induced male sterility exhibited in the P1 and F1 generation inLepidoptera. Radiat. Res.31, 615 (1967).
—, Holt, G. G.: Genetic and cytogenetic basis of radiation-induced sterility in the male cabbage looper,Trichoplusia ni. In: Isotopes and Radiation in Entomology. (Symposium Nov. 1967), p. 391–403. Vienna: Intern. Atomic Energy Agency 1968a.
— —: Inherited sterility in the progeny of irradiated male cabbage loopers, J. econ. Entomol.61, 928–931 (1968b).
Proshold, F. I., Bartell, J. A.: Inherited sterility in progeny of irradiated male tobacco budworms: Effects on reproduction, developmental time, and sex ratio. J. econ. Entomol. (in press).
Proverbs, M. D.: Progess on the use of induced sexual sterility for control of the codling moth,Carpocapsa pomonella L. Proc. entomol. Soc. Ontario92, 5–11 (1962).
Riemann, J. G.: Metamorphosis of sperm of the cabbage looper,Trichoplusia ni, during passage from the testes to the female spermathecae. Proc. of Symposium “Comparative Spermatology” held in Rome and Sienna, July 1–5, 1969 (in press).
Suomalainen, E.: The kinetochore and the bivalent structure in theLepidoptera. Hereditas (Lund)39, 88–96 (1953).
—: On the chromosomes of the geometrid moth genusCidaria. Chromosoma (Berl.)16, 166–184 (1965).
Ueshima, N.: Chromosome behavior of theCimex pilosellus complex (Cimicidae: Hemiptera). Chromosoma (Berl.)14, 511–521 (1963).
Virkki, N.: Gametogenesis in the sugarcane borer moth,Diatrea saccharalis (F.) (Crambidae). J. Agricult. Univ. Puerto Rico47, 98–137 (1963).
Walker, D. W., Quintana, V.: Inherited partial sterility among survivors from irradiation-eradication experiment. J. econ. Entomol.61, 318–319 (1968).
White, M. J. D.: Animal cytology and evolution, 2nd edit. 454 pp. Cambridge: Cambridge University Press 1954.
Wolfe, S. L., John, B.: The organization and ultrastructure of male meiotic chromosomes inOncopeltus fasciatus. Chromosoma (Berl.)17, 85–103 (1965).
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LaChance, L.E., Degrugillier, M. & Leverich, A.P. Cytogenetics of inherited partial sterility in three generations of the large milkweed bug as related to holokinetic chromosomes. Chromosoma 29, 20–41 (1970). https://doi.org/10.1007/BF01183659
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DOI: https://doi.org/10.1007/BF01183659