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High-affinity folate binding in human liver membranes

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Bioscience Reports

Abstract

High-affinity binding of3H-folate in Triton X-100 solubilized membranes of human liver displayed characteristics, e.g. apparent positive cooperativity, which are typical of specific folate binding. Ultrogel® AcA 44 chromatography of solubilized membranes saturated with3H-folate revealed a major peak of 100 kDa and a minor peak of 25 kDa. The 100 kDa peak could represent a hydrophobic membrane associated molecular form of the protein. This notion was supported by the fact that the two peaks had identical molecular weights as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis with immunoblotting.

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Authors and Affiliations

  1. Department of Clinical Chemistry, Central Hospital Nykøbing Falster, 4800, Nykøbing Falster, Denmark

    Jan Holm

  2. Department of Clinical Chemistry, Central Hospital Hillerød, 3400, Hillerød, Denmark

    Steen Ingemann Hansen

  3. Laboratory for Autoimmune Serology, State Serum Institute, 2300 S, Copenhagen, Denmark

    Mimi Høier-Madsen

Authors
  1. Jan Holm
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  2. Steen Ingemann Hansen
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  3. Mimi Høier-Madsen
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Holm, J., Hansen, S.I. & Høier-Madsen, M. High-affinity folate binding in human liver membranes. Biosci Rep 11, 139–145 (1991). https://doi.org/10.1007/BF01182482

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  • DOI: https://doi.org/10.1007/BF01182482

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